GLI Family Zinc Finger Protein 3 (AA 111-343) antibody

Catalog No. ABIN7439603

Product Details

Target: GLI Family Zinc Finger Protein 3

Binding Specificity: AA 111-343

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB), Immunocytochemistry (ICC)

Purpose: Polyclonal Antibody to GLI Family Zinc Finger Protein 3 (GLI3)

Specificity: The antibody is a rabbit polyclonal antibody raised against GLI3. It has been selected for its ability to recognize GLI3 in immunohistochemical staining and western blotting.

Cross-Reactivity: Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant GLI Family Zinc Finger Protein 3 (GLI3) corresdonding to Met111~Ser343 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL

Immunohistochemistry: 5-20 μg/mL

Immunocytochemistry: 5-20 μg/mL

Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details

Target: GLI Family Zinc Finger Protein 3

Background: GCPS, PHS, PAP-A, PAPA, PAPA1, PAPB, ACLS, PPDIV, Greig Cephalopolysyndactyly Syndrome, Glioma-Associated Oncogene Family Zinc Finger 3, GLI3 form of 190 kDa