ADRA1D antibody (Cys240Ser-Mutant, Extracellular)

Catalog No. ABIN7042896

Product Details anti-ADRA1D Antibody

Target: ADRA1D (Adrenergic Receptor, alpha 1d (ADRA1D))

Binding Specificity: AA 231-245, Cys240Ser-Mutant, Extracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ADRA1D antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF), Immunochromatography (IC), Live Cell Imaging (LCI), Immunofluorescence (Cultured Cells) (IF (cc))

Purpose: A Rabbit Polyclonal Antibody to α1D-Adrenergic Receptor

Specificity: 3rd extracellular loop

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat,human,mouse - 14,15 amino acid residues identical

Characteristics: Anti-alpha1D-Adrenergic Receptor (extracellular) Antibody is directed against an extracellular epitope of the rat α1D-adrenergic receptor. Anti-α1D-Adrenergic Receptor (extracellular) Antibody (ABIN7042896, ABIN7043916 and ABIN7043917)) can be used in western blot, indirect flow cytometry and immunohistochemical applications. It has been designed to recognize α1D-adrenoceptor from mouse, rat and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)EPVPPDERF*SGITEE, corresponding to amino acid residues 231-245 of rat alpha1D-adrenoceptor with replacement of cysteine 240 (C240) with serine (*S)

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: BLP-AR019

Blocking Peptide: BLP-AR019

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for ADRA1D

Target: ADRA1D (Adrenergic Receptor, alpha 1d (ADRA1D))

Alternative Name: ADRA1D (ADRA1D Products)

Background: Alpha-1D adrenoceptor, Alpha-1D adrenoreceptor, ADRA1D,Adrenergic receptors (also called adrenoceptors) are the receptors for the catecholamines adrenaline and noradrenaline (called epinephrine and norepinephrine in the United States). Adrenaline and noradrenaline play important roles in the control of blood pressure, myocardial contractile rate and force, airway reactivity, and a variety of metabolic and central nervous system functions. Adrenergic receptors are members of the G-protein coupled receptor (GPCR) superfamily of membrane proteins. They share a common structure of seven putative transmembrane domains, an extracellular amino terminus, and a cytoplasmic carboxyl terminus.Adrenoceptors are divided into three types: α1, α2 and β-adrenoceptors. Each type is further divided into at least three subtypes: α1A, α1B, α1D, α2A, α2B, α2C, β1, β2, β31,2. The adrenoceptors are expressed in nearly all peripheral tissues and in the central nervous system1,2. α1-Adrenergic receptors play an important role in the physiological response to epinephrine and norepinephrine, particularly in the cardiovascular system. All three cloned α1 receptors (α1A, α1B, and α1D) couple to Gq/11. While their cellular distribution is clear, their functional role is less so due to a lack of specific agonists/antagonists3. As these receptors lack specific agonists/antagonists, knock-out (KO) studies in mice have shed significant light on their cellular role. Individual α1-adrenoceptor KO mice do not display physical abnormalities in general. Similar KO studies indicate that all α1 subtypes seem to be involved in the regulation of blood pressure3. Also, studies indicate that α1D-adrenoceptor seems to play an important role in aortic contraction4, renal function, nociception, basal locomotor activity3 as well as bladder contraction5.

Alternative names: alpha1D-Adrenergic Receptor, Alpha-1D adrenoceptor, Alpha-1D adrenoreceptor, ADRA1D

Gene ID: 29413

NCBI Accession: NM_000678

UniProt: P23944