ADRA2B antibody (Cys169Ser-Mutant, Extracellular)

Catalog No. ABIN7042900

Product Details anti-ADRA2B Antibody

Target: ADRA2B (Adrenergic, alpha-2B-, Receptor (ADRA2B))

Binding Specificity: AA 160-174, Cys169Ser-Mutant, Extracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ADRA2B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (Cultured Cells) (IF (cc))

Purpose: A Rabbit Polyclonal Antibody to α2B-Adrenergic Receptor

Specificity: 2nd extracellular loop

Predicted Reactivity: 14 amino acid residues identical,Rat - 14,15 amino acid residues identical, human - 10, mouse - 13

Characteristics: Anti-alpha2B-Adrenergic Receptor (extracellular) Antibody is directed against an extracellular epitope of the rat α2B-adrenoceptor. Anti-α2B-Adrenergic Receptor (extracellular) Antibody (ABIN7042900, ABIN7043920 and ABIN7043921)) can be used in western blot and immunohistochemistry applications. It has been designed to recognize α2B-adrenoceptor from mouse, rat, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RPEPRGLPQ*SELNQE, corresponding to amino acid residues 160-174 of rat alpha2B-adrenoceptor with replacement of cysteine 169 (C169) with serine (*S)

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100-1:300

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: BLP-AR021

Blocking Peptide: BLP-AR021

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for ADRA2B

Target: ADRA2B (Adrenergic, alpha-2B-, Receptor (ADRA2B))

Alternative Name: ADRA2B (ADRA2B Products)

Background: Alpha-2B adrenoceptor, Alpha-2B adrenoreceptor, Alpha-2BAR, ADA2B, ADRA2B,Adrenergic receptors (also called adrenoceptors) are the receptors for the catecholamines adrenaline and noradrenaline (called epinephrine and norepinephrine in the United States). Adrenaline and noradrenaline play important roles in the control of blood pressure, myocardial contractile rate and force, airway reactivity, and a variety of metabolic and central nervous system functions.Adrenergic receptors are members of the G-protein coupled receptor (GPCR) superfamily of membrane proteins. They share a common structure of seven putative transmembrane domains, an extracellular amino terminus, and a cytoplasmic carboxyl terminus.Adrenoceptors are divided into three types: α1, α2 and β-adrenoceptors. Each type is further divided into at least three subtypes: α1A, α1B, α1D, α2A, α2B, α2C, β1, β2, β31,2. Adrenoceptors are expressed in nearly all peripheral tissues and in the central nervous system1,2. The α2B-adrenoceptor has a distinct pattern of expression within the brain, liver lung and kidney, and recent studies using the knock out mouse system have shown that disruption of this receptor indeed affects mouse viability3, blood pressure responses to α2-adrenoceptor agonists3 and the hypertensive response to salt loading4. Like the α2A-adrenoceptor subtype, the α2B-adrenoceptor undergoes short term agonist promoted desensitization5. This desensitization is due to the phosphorylation of the receptor by G-protein coupled receptor kinases (GRKs)6 which ultimately promotes uncoupling of the receptor from the G-protein subunit7.

Alternative names: alpha2B-Adrenergic Receptor, Alpha-2B adrenoceptor, Alpha-2B adrenoreceptor, Alpha-2BAR, ADA2B, ADRA2B

Gene ID: 24174

NCBI Accession: NM_000682

UniProt: P19328

Pathways: EGFR Signaling Pathway, cAMP Metabolic Process