ADRB3 antibody (Cys186Ser-Mutant, Extracellular)

Catalog No. ABIN7042907

Product Details anti-ADRB3 Antibody

Target: ADRB3 (Adrenergic, beta-3-, Receptor (ADRB3))

Binding Specificity: AA 180-192, Cys186Ser-Mutant, Extracellular

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ADRB3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (IF)

Purpose: A Rabbit Polyclonal Antibody to β3-Adrenergic Receptor

Specificity: 2nd extracellular loop

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat - 12,13 amino acid residues identical, human - 11

Characteristics: Anti-beta3-Adrenergic Receptor (extracellular) Antibody is directed against an extracellular epitope of the mouse β3-adrenoceptor. Anti-β3-Adrenergic Receptor (extracellular) Antibody (ABIN7042907, ABIN7043912 and ABIN7043913) can be used in western blot and immunohistochemical applications. It has been designed to recognize both splice variants of the β3AR from mouse, rat and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: DAEAQESH*SNPRC, corresponding to amino acid residues 180-192 of mouse ADRB3 with replacement of cysteine 186 (C186) with serine (*S)

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: (ABIN7234680)

Blocking Peptide: (ABIN7234680)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for ADRB3

Target: ADRB3 (Adrenergic, beta-3-, Receptor (ADRB3))

Alternative Name: ADRB3 (ADRB3 Products)

Background: Beta-3 adrenoceptor, Beta-3 adrenoreceptor, ADRB3, B3AR,Adrenergic receptors (also called adrenoceptors) are the receptors for the catecholamines adrenaline and noradrenaline (called epinephrine and norepinephrine in the United States). Adrenaline and noradrenaline play important roles in the control of blood pressure, myocardial contractile rate and force, airway reactivity, and a variety of metabolic and central nervous system functions.Adrenergic receptors are members of the G-protein coupled receptor (GPCR) superfamily of membrane proteins. They share a common structure of seven putative transmembrane domains, an extracellular amino terminus, and a cytoplasmic carboxyl terminus.Adrenoceptors are divided into three types: α1, α2 and β-adrenoceptors. Each type is further divided into at least three subtypes: α1A, α1B, α1D, α2A, α2B, α2C, β1, β2, β3. 1,2 Adrenoceptors are expressed in nearly all peripheral tissues and in the central nervous system.1,2 The β3-adrenoceptor is insensitive to the commonly used β-antagonists and has often been referred to as the 'atypical' β-adrenoceptor. Mouse β3-adrenoceptor has two known splice variants, isoforms β3a-adrenoceptor and β3b-adrenoceptor. Both isoforms are expressed in white and brown adipose tissues.3 The β3b-adrenoceptor is highly expressed in brain.The β3- adrenoceptor has been found to play a key role in the lipolytic action of catecholamines.4

Alternative names: beta3-Adrenergic Receptor, Beta-3 adrenoceptor, Beta-3 adrenoreceptor, ADRB3, B3AR

Gene ID: 11556

NCBI Accession: NM_000025

UniProt: P25962

Pathways: cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Feeding Behaviour, Brown Fat Cell Differentiation