Aquaporin 5 antibody (Intracellular)

Catalog No. ABIN7042941

Product Details anti-Aquaporin 5 Antibody

Target: Aquaporin 5 (AQP5)

Binding Specificity: AA 251-265, Intracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Aquaporin 5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC)

Purpose: A Rabbit Polyclonal Antibody to Aquaporin 5 Channel

Specificity: Intracellular, C-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse - identical, human - 10,16 amino acid residues identical

Characteristics: Anti-Aquaporin 5 Antibody (ABIN7042941, ABIN7045200 and ABIN7045201)) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunocytochemistry and immunohistochemistry applications. It has been designed to recognize AQP5 from mouse, rat, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)DHREERKKTIELTAH corresponding to amino acid residues 251-265 of rat AQP5

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IHC|ICC|Whole Tissue Staining

Negative Control: BLP-QP005

Blocking Peptide: BLP-QP005

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Aquaporin 5

Target: Aquaporin 5 (AQP5)

Alternative Name: AQP5 (AQP5 Products)

Background: AQP5,The importance of the aquaporin water channels was underscored by awarding the 2003 Nobel Prize in Chemistry to Peter Agre "for the discovery of water channels". Today, eleven mammalian proteins that belong to the aquaporin family have been identified. The proteins present a conserved structure of six transmembrane domains with intracellular N- and C-termini. Aquaporin 5 (AQP5) is expressed in secretory glands, lung and eye, where it works in modulating the rate of fluid release. Since a number of pathological conditions including pulmonary edema, respiratory distress syndrome and congestive heart failure are characterized by disrupted fluid transport, it has been suggested that modulation of the expression of AQP5 could be a novel modality of treatment of the above illnesses. Indeed, several studies have shown that AQP5 expression is downregulated in animal models of acute lung infection.

Alternative names: Aquaporin 5, AQP5

Gene ID: 25241

NCBI Accession: NM_001651

UniProt: P47864