Aquaporin 8 antibody (Intracellular)

Catalog No. ABIN7042944

Product Details anti-Aquaporin 8 Antibody

Target: Aquaporin 8 (AQP8)

Binding Specificity: AA 14-28, Intracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Aquaporin 8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Purpose: A Rabbit Polyclonal Antibody to Aquaporin 8 Channel

Specificity: Intracellular, N-terminus

Predicted Reactivity: Unlikely to recognize mouse,human samples

Characteristics: Anti-Aquaporin 8 Antibody (ABIN7042944 and ABIN7045205)) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize AQP8 from rat samples only.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)REIKGKETNMADSYH, corresponding to amino acid residues 14-28 of rat AQP8

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100

Application Dilutions Western blot wb: 1:200-1:400

Comment:

Cited Application: IHC

Negative Control: BLP-QP008

Blocking Peptide: BLP-QP008

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Aquaporin 8

Target: Aquaporin 8 (AQP8)

Alternative Name: AQP8 (AQP8 Products)

Background: AQP8,Aquaporin 8 (AQP-8) belongs to a family of membrane proteins that allow passage of water and certain other solutes through biological membranes.1 The family is composed of 13 members (AQP-0 to AQP-12). Little is known about the function of the two newest members, AQP-11 and AQP-12.The aquaporins can be divided into two functional groups based on their permability characteristics: the aquaporins that are permeated primarily by water and include besides AQP-8, AQP-1, AQP-2, AQP4 and AQP-5, and the aquaglyceroporins that are permeated by water and other small solutes such as glycerol. This last group includes AQP-3, AQP-7, AQP-9 and AQP-10.The proteins present a conserved structure of six transmembrane domains with intracellular N- and C-termini. The functional channel is a tetramer but each subunit has a separate pore and therefore the functional channel unit, contains four pores.2AQP-8 is expressed along the gastrointestinal tract including small intestine, colon, pancreas and liver and also in other organs such as testis, lung and kidney.The physiological function of AQP-8 is not entirely clear but studies suggest that it has an important role in colonic water adsorption and hepatocyte bile formation.3,4 Interestingly, AQP-8 expression is markedly altered in gastrointestinal disorders such as ulcerative colitis and inflammatory bowel disease.5

Alternative names: Aquaporin 8, AQP8

Gene ID: 29172

NCBI Accession: NM_001169

UniProt: P56405