Bestrophin 2 antibody (Extracellular)

Catalog No. ABIN7042965

Product Details anti-Bestrophin 2 Antibody

Target: Bestrophin 2 (BEST2)

Binding Specificity: AA 259-270, Extracellular

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Bestrophin 2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Purpose: A Rabbit Polyclonal Antibody to Bestrophin-2 Channel

Specificity: 3rd extracellular loop

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat - identical, human - 11,12 amino acid residues identical

Characteristics: Anti-Bestrophin-2 (extracellular) Antibody is directed against an epitope located at the 3rd extracellular loop of mouse Bestrophin-2. Anti-Bestrophin-2 (extracellular) Antibody (ABIN7042965 and ABIN7043936)) can be used in western blot and immunohistochemical applications, and has been designed to recognize bestrophin-2 channel from mouse, rat and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)DPAQGYKDHTLD, corresponding to amino acid residues 259-270 of mouse Bestrophin-2

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IHC

Negative Control: BLP-BC002

Blocking Peptide: BLP-BC002

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Bestrophin 2

Target: Bestrophin 2 (BEST2)

Alternative Name: BEST2 (BEST2 Products)

Background: BEST2, Vitelliform macular dystrophy 2-like protein 1, VMD2L1,Mammalian Cl- channels can be broadly classified into four different families: voltage-dependent Cl- channels (CLCs), the cystic fibrosis transmembrane conductance regulator (CFTR), ligand-gated Cl- channels (γ-aminobutyric acid (GABA)) and glycine channels) and Ca2+-activated Cl- channels (Bestrophin and Anoctamin channels).Bestrophins were first found by genetic linkage of human-Bestrophin-1 (hBest1) to a juvenile form of macular degeneration called Best vitelliform macular dystrophy1,2. To date Bestrophin 1-4 have been identified, although Bestrophin-3 and Bestrophin-4 have been observed only at the RNA level3. In addition, splice variants of some of these Ca2+-activated Cl- channels (CaCCs) have also been detected2,4,5. CaCCs are known to be involved in the regulation of olfaction, taste, phototransduction, and excitability in the nervous system. However, the molecular identity and functional role of CaCC in the brain have not been well established6. Bestrophin-2 is prominently expressed in colon and testes. Recently, Bestrophin-2 has also been found to be expressed in olfactory sensory neurons (OSNs)7.Two different topologies for Bestrophin-1 have been proposed. The first structure proposes that six hydrophobic domains span the membrane8, while the second suggests that there are only four membrane-spanning domains9. The same structural controversy applies to Bestrophin-2. Bestrophin-1 and Bestrophin-2 (as well as Bestrophin-4 by heterologous expression) are activated by intracellular Ca2+ 10-12. Ca2+-dependent activation of Bestrophin-2 was also demonstrated in Xenopus13.

Alternative names: Bestrophin-2, BEST2, Vitelliform macular dystrophy 2-like protein 1, VMD2L1

Gene ID: 212989

NCBI Accession: NM_017682

UniProt: Q8BGM5