BRS3 antibody (Extracellular)

Catalog No. ABIN7042966

Product Details anti-BRS3 Antibody

Target: BRS3 (Bombesin-Like Receptor 3 (BRS3))

Binding Specificity: AA 186-201, Extracellular

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BRS3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (Cultured Cells) (IF (cc)), Live Cell Imaging (LCI)

Purpose: A Rabbit Polyclonal Antibody to Bombesin Receptor 3

Specificity: 2nd extracellular loop

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat,mouse - 13,16 amino acids residues identical

Characteristics: Anti-Bombesin Receptor 3 (extracellular) Antibody is directed against a well conserved epitope of the human BB3 receptor. Anti-Bombesin Receptor 3 (extracellular) Antibody (ABIN7042966 and ABIN7043938)) can be used in western blot analysis, as well as immunohistochemical and flow cytometry applications, and will recognize BRS3 from rat and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)SNVYTFRDPNKNMTFE, corresponding to amino acid residues 186-201 of human BRS3 

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:50

Application Dilutions Western blot wb: 1:200-1:500

Comment:

Negative Control: BLP-BR003

Blocking Peptide: BLP-BR003

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for BRS3

Target: BRS3 (Bombesin-Like Receptor 3 (BRS3))

Alternative Name: BRS3 (BRS3 Products)

Background: BB3R, BRS3,Bombesin receptor 3 (BB3) is a member of a family of receptors that binds the 14 amino acid peptide bombesin. Bombesin was first isolated from frog skin and it was later established that mammalians express endogenous bombesin like peptides such as gastrin-releasing peptide (GRP) a 27 amino acid homologue and neuromedin B (NMB) a 10 amino acid homologue.1BB1 is the preferred receptor for NMB while BB2 is the preferred receptor for GRP.BB3 was the third member of the bombesin receptors family to be cloned based in its sequence similarity to BB1 and BB2.2 The affinity of BB3 for bombesin is lower than that of the BB1 and BB2 receptors as is its affinity for NMB and GRP. This suggests that the mammalian endogenous ligand for the BB3 receptor remains to be identified.All three bombesin receptors are members of the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily. BB3 is coupled to a Gq/11 protein that activates phospholipase C (PLC) and leads to production of inositol 1,4,5-trisphosphate (InsP3), intracellular Ca2+ mobilization and cell growth.3The physiological function of the BB3 receptor is not clear. However, studies with BB3 receptor deficient mice have shown that the mice develop mild obesity associated with hypertension and reduced metabolic rate.4,5Finally, BB3 receptor expression has been identified in several human tumors most notably lung. BB3 is therefore considered a potential target for the development of both diagnostics and anti-cancer therapies.6

Alternative names: Bombesin Receptor 3, BB3, BRS3

Gene ID: 680

NCBI Accession: NM_001727

UniProt: P32247

Pathways: Feeding Behaviour