CHERP antibody (Cytosolic)

Catalog No. ABIN7043011

Product Details anti-CHERP Antibody

Target: CHERP (Calcium Homeostasis Endoplasmic Reticulum Protein (CHERP))

Binding Specificity: AA 669-684, Cytosolic

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHERP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purpose: A Rabbit Polyclonal Antibody to CHERP

Specificity: Cytoplasmic

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat,human - identical

Characteristics: Anti-CHERP Antibody (ABIN7043011, ABIN7044119 and ABIN7044120)) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize CHERP from human, rat, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KLEDHEYKPLDPKDIR, corresponding to amino acid residues 669-684 of mouse CHERP

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:1500

Comment:

Negative Control: BLP-CC330

Blocking Peptide: BLP-CC330

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for CHERP

Target: CHERP (Calcium Homeostasis Endoplasmic Reticulum Protein (CHERP))

Alternative Name: CHERP (CHERP Products)

Background: Calcium homeostasis endoplasmic reticulum protein, SR-related CTD-associated factor 6, SCAF6,CHERP (calcium homeostasis endoplasmic reticulum protein) is a ubiquitously expressed integral sarco/endoplasmic reticulum (SR/ER) membrane protein. CHERP protein consist of 916 amino acid in humans and its structure containing a Pro-rich region and a segment of Arg-Ser dipeptide repeats (an RS domain) near the C terminus, and an RNA recognition motifs (RRMs)1-3.CHERP acts as a regulator of both major families of endoplasmic reticulum Ca2+ channels, inositol 1,4,5-trisphosphate receptors (IP3Rs) and ryanodine receptors (RyRs). CHERP is responsible for the regulation of intracellular Ca2+ mobilization in human cells and is involved in neuroblastoma cell proliferation, apoptosis and tumorigenicity1,2.CHERP co-localizes with IP3 receptors throughout the cytoplasmic and perinuclear regions in human erythroleukemia cells and in Jurkat cells. CHERP is also detected in the nucleoplasm with prominent accumulation at nuclear speckles1,2. CHERP co-localizes with endogenous ryanodine receptor type 1 (RyR1) in the sarcoplasmic reticulum of rat soleus muscle. Studies of CHERP knockdown reveal decreased intracellular Ca2+ mobilization, cell growth and proliferation2,3.

Alternative names: CHERP, Calcium homeostasis endoplasmic reticulum protein, SR-related CTD-associated factor 6, SCAF6

Gene ID: 27967

NCBI Accession: NM_006387

UniProt: Q8CGZ0