CNR2 antibody (Extracellular)

Catalog No. ABIN7043026

Product Details anti-CNR2 Antibody

Target: CNR2 (Cannabinoid Receptor 2 (CNR2))

Binding Specificity: AA 11-24, Extracellular

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CNR2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunofluorescence (Cultured Cells) (IF (cc)), Live Cell Imaging (LCI), Immunochromatography (IC)

Purpose: A Rabbit Polyclonal Antibody to Cannabinoid Receptor 2

Specificity: Extracellular, N-terminus

Cross-Reactivity (Details): Not recommended to use with rat and mouse samples.

Predicted Reactivity: Canis,bovine - 10,14 amino acid residues identical

Characteristics: Anti-Human Cannabinoid Receptor 2 (extracellular) Antibody is directed against an extracellular epitope of the human CB2 receptor. Anti-Human Cannabinoid Receptor 2 (extracellular) Antibody (ABIN7043026, ABIN7044159 and ABIN7044160)) can be used in western blot, immunohistochemistry and live cell imaging applications. It has been designed to recognize CB2 from human samples. The antibody is not recommended for use on rat and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)NGSKDGLDSNPMKD, corresponding to amino acid residues 11-24 of human CB2 receptor

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: BLP-CR003

Blocking Peptide: BLP-CR003

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for CNR2

Target: CNR2 (Cannabinoid Receptor 2 (CNR2))

Alternative Name: CNR2 (CNR2 Products)

Background: CB2, CNR2,Cannabinoids have been used as pain relievers in Eastern medicine for many years.1 To date, two specific cannabinoid receptors have been identified: cannabinoid receptor 1 (CB1) and cannabinoid receptor 2 (CB2).2 The cannabinoid receptors can be distinguished by their amino acid sequence, signaling mechanisms and tissue distribution.2 Both receptors belong to the G-protein coupled receptor superfamily and are coupled to Gi/0 G protein.2,3The CB2 is highly expressed in cells of the immune system such as macrophages, lymphocytes natural killer cells and mast cells but has also been shown to be expressed, by both, in situ-hybridization and in immunohistochemistry, in spleen, thymus, and pancreas.1,2,4 CB2 expression in brain is still much less characterized than that of CB1. Recently, it was demonstrated that CB2 is expressed in the brain and might have a role in controlling fundamental processes such as proliferation and survival of neural cells.5,6Overexpression of CB2 was reported in several cancers such as prostate, glioma and acute myeloid leukemias.7-9 In human astrocytoma a direct relation between CB2 expression and tumor malignancy was demonstrated.8 Activation of CB2R in vivo by its agonist JWH-133, completely blocked cell growth.8 In C6 glioma, it was shown that activation of the CB2 by JWH-133 resulted in internalization of only the CB2 and not CB1 leading to apoptosis of the cells. This may well be a new approach for the treatment of glioma.

Alternative names: Cannabinoid Receptor 2, CB2, CNR2

Gene ID: 1269

NCBI Accession: NM_001841

UniProt: P34972