HCRTR2 antibody (Intracellular)

Catalog No. ABIN7043029

Product Details anti-HCRTR2 Antibody

Target: HCRTR2 (Hypocretin (Orexin) Receptor 2 (HCRTR2))

Binding Specificity: AA 391-405, Intracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HCRTR2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC)

Purpose: A Rabbit Polyclonal Antibody to Orexin Receptor 2

Specificity: Intracellular, C-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse - identical, human - 14,15 amino acid residues identical

Characteristics: Anti-Orexin Receptor 2 Antibody (ABIN7043029, ABIN7044885 and ABIN7044886)) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize OX2R from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)DRLARGRTSTESRKS, corresponding to amino acid residues 391-405 of rat OX2R

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:50

Application Dilutions Western blot wb: 1:200-1:400

Comment:

Cited Application: IFC

Negative Control: BLP-OR002

Blocking Peptide: BLP-OR002

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for HCRTR2

Target: HCRTR2 (Hypocretin (Orexin) Receptor 2 (HCRTR2))

Alternative Name: HCRTR2 (HCRTR2 Products)

Background: OX2R, Hypocretin receptor type 2, HCRTR2,Orexin Receptor 2 (OX2R) (also known as hypocretin receptor 2) is one of two receptors that recognize the peptide neurotransmitters orexin A and orexin B.1 Orexin A and B are 33 and 28 amino acids in length, respectively, and are derived from a common precursor termed prepro-orexin.OX2R binds both orexin A and B with similar affinities while OX1R binds orexin A with greater affinity than orexin B (a one order of magnitude difference).2,3Both OX2R and OX1R belong to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily. OX2R is thought to transmit signals through the Ga11 class of G proteins, resulting in the activation of phospholipase C with subsequent triggering of the phosphatidylinositol cascade and an influx of extracellular Ca2+, probably through transient receptor potential (TRP) channels. OX2R can also transmit signals through inhibitory Gi G proteins, although the mechanism is less well understood.2,3The physiological functions of the orexin system (OX1R, OX2R, and their ligands) have been a matter of intense research over the last few years. OX2R is expressed in both the central nervous system and peripheral locations such as gastrointestinal tissues, pancreas, and testis.2The best studied physiological role of OX2R is its involvement in the regulation of sleep and wakefulness states. Studies in mice lacking the orexin gene and dogs expressing a null mutation of the OX2R show a remarkably similar phenotype to human narcolepsy, a condition characterized by excessive daytime sleepiness, inability to maintain vigilant states, and defects in the regulation of rapid eye movement (REM) sleep.4In addition, the orexin system is involved in regulating autonomic functions such as blood pressure and heart rate, as well as in mechanisms that regulate the reward response in the brain.4

Alternative names: Orexin Receptor 2, OX2R, Hypocretin receptor type 2, HCRTR2

Gene ID: 25605

NCBI Accession: NM_001384272

UniProt: P56719

Pathways: Feeding Behaviour