PAR1 antibody (Extracellular)

Catalog No. ABIN7043073

Product Details anti-PAR1 Antibody

Target: PAR1 (F2R) (Coagulation Factor II (thrombin) Receptor (F2R))

Binding Specificity: AA 61-76, Extracellular

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAR1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (IF), Live Cell Imaging (LCI), Immunochromatography (IC)

Purpose: A Rabbit Polyclonal Antibody to Protease-Activated Receptor-1

Specificity: Extracellular, N-terminus

Cross-Reactivity (Details): Unlikely to recognize mouse or rat samples.

Predicted Reactivity: Monkey - identical

Characteristics: Anti-Human PAR1 (F2R) (extracellular) Antibody (ABIN7043073, ABIN7045102 and ABIN7045103)) is a highly specific antibody directed against an epitope of human protease-activated receptor-1. The antibody can be used in western blot, immunohistochemistry, immunocytochemistry, and live cell flow cytometry applications. It has been designed to recognize PAR-1 from human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KNESGLTEYRLVSINK, corresponding to amino acid residues 61-76 of human PAR-1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: ICC

Negative Control: BLP-PR031

Blocking Peptide: BLP-PR031

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for PAR1

Target: PAR1 (F2R) (Coagulation Factor II (thrombin) Receptor (F2R))

Alternative Name: F2R (F2R Products)

Background: Protease-activated receptor-1, PAR-1, Thrombin receptor, Coagulation factor II receptor, CF2R,Protease-activated receptor 1 (PAR-1) belongs to a family of four G protein-coupled receptors (PAR1-4) that are activated as a result of proteolytic cleavage by certain serine proteases, hence their name. In this novel modality of activation, a specific protease cleaves the PAR receptor within a defined sequence in its extracellular N-terminal domain. This results in the creation of a new N-terminal tethered ligand, which subsequently binds to a site in the second extracellular loop of the same receptor. This binding results in the coupling of the receptor to G proteins and in the activation of several signal transduction pathways.1-3Different PARs are activated by different proteases. Hence, PAR-1 is activated by thrombin (and is in fact also known as the thrombin receptor), as are PAR-3 and PAR-4, while PAR-2 is activated by trypsin.1-3 PAR-1 can be also cleaved and activated by other proteases such as plasmin, Factor Xa, cathepsin G, and others.The intramolecular nature of PAR activation and the continuous presence of the tethered ligand that cannot diffuse away imply the existence of several mechanisms for the rapid termination of PAR signaling. Indeed, following receptor activation, there is rapid phosphorylation of the C-terminal end of the receptor, followed by receptor internalization and degradation. In addition, several proteases can cleave away the tethered ligand, thereby "disarming" the PAR.1-3PAR-1 signals through several G proteins including Gaq, Gai, and Ga12/13, resulting in the activation of several transduction pathways including intracellular Ca2+ mobilization, Rho and Rac signaling, and MAPK activation.1-3PAR-1 is expressed in several cell types including platelets, leukocytes, vascular endothelial cells, gastrointestinal epithelial cells, myocytes, and neurons. The best studied physiological function of PAR-1 is its involvement in the coagulation cascade. Thrombin, the preeminent ligand of PAR-1, activates the receptor on the surface of platelets, hence inducing platelet aggregation, granular secretion, and procoagulant activity. PAR-1 also plays a crucial role in vascular ontogenesis. Accordingly, PAR-1 knockout mice show bleeding at multiple sites and usually die at mid-gestation.1-3PAR-1 also plays important roles in tumor growth and metastasis. PAR-1 is upregulated in several human cancers as are several proteases such as plasmin and matrix metalloproteases (MMPs) that act as PAR-1 ligands, thereby creating an autocrine loop. PAR-1 activation in cancer cells transmits mitogenic signals through the activation of the erk1/2 pathway and is involved in tumor spread via its pro-angiogenic activity.4

Alternative names: PAR1 (F2R), Protease-activated receptor-1, PAR-1, Thrombin receptor, Coagulation factor II receptor, CF2R

Gene ID: 2149

NCBI Accession: NM_001992

UniProt: P25116

Pathways: Nuclear Receptor Transcription Pathway, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus