GPR55 antibody (Extracellular)
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- Target See all GPR55 Antibodies
- GPR55 (G Protein-Coupled Receptor 55 (GPR55))
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Binding Specificity
- AA 261-275, Extracellular
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Reactivity
- Mouse
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This GPR55 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (Cultured Cells) (IF (cc))
- Purpose
- A Rabbit Polyclonal Antibody to GPR55
- Specificity
- 3rd extracellular loop
- Cross-Reactivity
- Mouse, Rat
- Cross-Reactivity (Details)
- Not recommended for use with human samples.
- Predicted Reactivity
- rat - 11,15 amino acid residues identical,Mouse - 14
- Characteristics
- Anti-GPR55 (extracellular) Antibody (ABIN7043162, ABIN7044202 and ABIN7044203)) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and live cell flow cytometry applications. It has been designed to recognize GPR55 from human, rat, and mouse samples. The antibody will not recognize the human protein.
- Purification
- Affinity purified on immobilized antigen.
- Immunogen
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Immunogen: Synthetic peptide
Immunogen Sequence: (C)RNRFILD(S)RMKQGIS, corresponding to amino acid residues 261 - 275 of mouse GPR55
- Isotype
- IgG
- Top Product
- Discover our top product GPR55 Primary Antibody
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- Application Notes
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A
Application Dilutions Western blot wb: N/A
- Comment
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Negative Control: BLP-CR062
Blocking Peptide: BLP-CR062
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- 0.2 mL double distilled water (DDW).
- Concentration
- 1 mg/mL
- Buffer
- PBS pH 7.4
- Storage
- 4 °C,-20 °C
- Storage Comment
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- Target
- GPR55 (G Protein-Coupled Receptor 55 (GPR55))
- Alternative Name
- GPR55 (GPR55 Products)
- Background
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G-Protein Coupled Receptor 55,G protein coupled receptor 55 (GPR55) is a cannabinoid receptor activated by a number of endo-, phyto- and synthetic cannabinoid ligands such as THC, JWH015 and anandamide1-3.GPR55 is only 13.5 % identical to the canonic cannabinoid receptor CB1 and 14.4 % identical to CB2. Upon ligand binding, it initiates a signaling cascade quite distinct from those used by CB1 and CB2 that eventually leads to an increase in intracellular calcium levels. GPR55 belongs to the Class A GPCRs. It shares many similarities with rhodopsin and as all other G-protein coupled receptors in its basic structure of seven transmembrane domains, intracellular C- terminus and extracellular N-terminal extensions4. The protein is highly expressed in large dorsal root ganglion neurons and its mRNA is present in the brain and periphery1.GPR55 has a role in self-renewing adult neural stem cell development and in hippocampal neurogenesis3. Experiments with mice in which GPR55 has been inactivated reveal a role for this receptor in neuropathic and inflammatory pain as well as in bone physiology. Thus deciphering the pharmacology of this receptor could lead to new therapeutics in these fields5.
Alternative names: G-Protein Coupled Receptor 55 - Gene ID
- 227326
- NCBI Accession
- NM_005683
- UniProt
- Q3UJF0
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