GABBR1 antibody (Extracellular)

Catalog No. ABIN7043195

Product Details anti-GABBR1 Antibody

Target: GABBR1 (gamma-aminobutyric Acid (GABA) B Receptor, 1 (GABBR1))

Binding Specificity: AA 66-79, Extracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GABBR1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunofluorescence (Cultured Cells) (IF (cc)), Live Cell Imaging (LCI)

Purpose: A Rabbit Polyclonal Antibody to GABA(B) Receptor 1

Specificity: Extracellular, N-terminus

Cross-Reactivity: Human, Mouse, Rat

Cross-Reactivity (Details): Does not recognize alternative splice 1b.

Predicted Reactivity: Mouse,human,canine,bovine - identical

Characteristics: Anti-GABA(B) R1 (extracellular) Antibody is directed against an epitope of rat GABA(B) receptor 1. Anti-GABA(B) R1 (extracellular) Antibody (ABIN7043195, ABIN7044319 and ABIN7044320)) can be used in western blot and immunohistochemistry applications. It has been designed to recognize GABBR1 from human, rat, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: CRGEREVVGPKVRK, corresponding to amino acid residues 66-79 of rat GABBR1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: BLP-GB001

Blocking Peptide: BLP-GB001

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for GABBR1

Target: GABBR1 (gamma-aminobutyric Acid (GABA) B Receptor, 1 (GABBR1))

Alternative Name: GABBR1 (GABBR1 Products)

Background: γ-Aminobutyric acid type B receptor subunit 1, GABA B receptor 1, GABABR1, Gb1, GABBR1, GPRC3A,GABA (gamma-aminobutyric acid) is the major inhibitory neurotransmitter in the central nervous system and plays a crucial role in the modulation of neuronal activity.1,2The GABA transmitter interacts with three types of receptors: the ionotropic receptors, GABA(A) R and GABA(C) R, and the metabotropic GABA (B) receptor, [GABA(B) R].3 The latter belongs to the G-protein coupled receptor superfamily and mediates slow synaptic inhibition in the brain and spinal cord.1,4The functional GABA (B) receptor is a heterodimer consisting of two subunits, the GABA(B) R1 and the GABA(B) R2. These subunits were demonstrated to have complementary roles essential for the functional receptor.5,6 The GABA(B) R1 subunit was demonstrated to be important for agonist and antagonist binding, while GABA(B) R2 was shown to be essential for trafficking and for G-protein binding.To date, eight alternatively spliced isoforms of GABA (B) R1 have been proposed. These are named 1a-1h, of which 1a and 1b are the most prominent. Only 1a, 1b, and 1c appear to act as functional subunits.6,7 They are widespread, being expressed in the testis, stomach, spinal cord, and brain. Isoform 1b is also expressed in the kidney and liver.7

Alternative names: GABA(B) R1, gamma-Aminobutyric acid type B receptor subunit 1, GABA B receptor 1, GABABR1, Gb1, GABBR1, GPRC3A

Gene ID: 81657

NCBI Accession: NM_001319053

UniProt: Q9Z0U4

Pathways: Positive Regulation of Peptide Hormone Secretion, cAMP Metabolic Process