GJA4 antibody (AA 131-144)

Catalog No. ABIN7043200

Product Details anti-GJA4 Antibody

Target: GJA4 (Gap Junction Protein, alpha 4, 37kDa (GJA4))

Binding Specificity: AA 131-144

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GJA4 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: A Rabbit Polyclonal Antibody to Connexin-37

Specificity: Intracellular loop

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse - identical, human - 13,14 amino acid residues identical

Characteristics: Anti-Connexin-37 Antibody is directed against an epitope of rat Connexin-37. Anti-Connexin-37 Antibody (ABIN7043200, ABIN7044081 and ABIN7044082)) can be used in western blot analysis. It has been designed to recognize Cx37 from rat, mouse and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)EHQMAKISVAEDGR, corresponding to amino acid residues 131-144 of rat Connexin-37

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200-1:400

Comment:

Negative Control: BLP-CC204

Blocking Peptide: BLP-CC204

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for GJA4

Target: GJA4 (Gap Junction Protein, alpha 4, 37kDa (GJA4))

Alternative Name: GJA4 (GJA4 Products)

Background: CX37, Gap junction α4 protein, GJA4,Connexins (Cxs) are a family of transmembrane proteins comprising 21 members in humans that forms gap junction channels (GJCs). Connexins are divided in four groups: α, β, γ and δ. Cx37 is also named α4 since it is the fourth connexin of the alpha group that was identified1,2. Connexins do not function as single proteins, six connexins assemble into connexons (hemi-channels), and two connexons from neighboring cells dock in the extracellular space to form a full gap junction channel. Connexins are responsible for mediating direct communication between adjacent cells by the exchange of ions and small signaling molecules and cytosolic factors1,3. Their structure consists of four transmembrane helices, two extracellular loops, a cytoplasmic loop, a cytoplasmic NH2- and COOH-termini1,2.Connexin-37 is encoded by the GJA4 gene and is expressed in vascular endothelial cells. The protein is responsible for regulating and controlling the growth, proliferation and regeneration of the vascular endothelium by mediating the signal transduction between smooth muscle and endothelial cells. Cx37 knockout mice have a tendency for developing endarterium atheromatous plaques4. Also, a polymorphism in the gene that encodes for Cx37, is associated with increased risk for atherosclerosis and myocardial infarction1.

Alternative names: Connexin-37, CX37, Gap junction alpha4 protein, GJA4

Gene ID: 25655

NCBI Accession: NM_002060

UniProt: Q03190

Pathways: Cell-Cell Junction Organization