GLRA4 antibody (Extracellular)

Catalog No. ABIN7043259

Product Details anti-GLRA4 Antibody

Target: GLRA4 (Glycine Receptor, alpha 4 (GLRA4))

Binding Specificity: AA 28-42, Extracellular

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLRA4 antibody is un-conjugated

Application: Immunofluorescence (IF), Live Cell Imaging (LCI), Western Blotting (WB), Immunochromatography (IC)

Purpose: A Rabbit Polyclonal Antibody to Glycine Receptor α4

Specificity: Extracellular, N-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: human - 13,Rat - 14,15 amino acid residues identical

Characteristics: Anti-Glycine Receptor alpha4 (extracellular) Antibody is directed against an extracellular epitope of the mouse glycine receptor α4. Anti-Glycine Receptor α4 (extracellular) Antibody (ABIN7043259, ABIN7044404 and ABIN7044405)) can be used in western blot and live cell imagine applications. The antibody recognizes an extracellular epitope and thus is ideal for detecting GlyRα4 in living cells. It has been designed to recognize GlyRα4 from human, rat and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KEDVKSGLKGSQPMS, corresponding to amino acid residues 28-42 of mouse GlyRalpha4

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:500

Comment:

Negative Control: BLP-GR015

Blocking Peptide: BLP-GR015

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for GLRA4

Target: GLRA4 (Glycine Receptor, alpha 4 (GLRA4))

Alternative Name: GLRA4 (GLRA4 Products)

Background: Glycine receptor subunit alpha-4, GLRA4,The amino acid glycine mediates neuronal inhibition by activating glycine receptors (GlyRs), which are ligand-gated chloride channels of the nicotinic acetylcholine receptor superfamily. GlyRs are known to mediate postsynaptic inhibition in spinal cord, brain stem and some higher brain regions1. GlyR is a member of the pentameric Cys-loop ion channel receptor family. Functional Cys-loop receptors comprise homomeric or heteromeric pentameric oligomers with each of the five subunits arranged symmetrically in a ring around a central ion-conducting pore. Several developmentally and regionally regulated GlyR isoforms exist, which result from a differential expression of the GlyR α (α1- α4) and β subunit genes. Each GlyR subunit comprises a large extracellular amino-terminal domain that harbors the ligand-binding sites and the eponymous Cys-loop. This connects to a bundle of four α-helical transmembrane domains (labelled M1-M4) with a large intracellular domain between M3 and M4 and a short extracellular C-terminal tail2. The human α4 subunit is considered a pseudogene on the grounds that it incorporates a premature stop codon upstream of the final TM4 domain3.The α4 subunit has been found in the adult mouse retina where it clusters together with synaptic markers such as bassoon or gephyrin. Furthermore, GlyR α4 transcripts have been detected in the white matter tract of the developing spinal cord from mice. The avian α4 mRNA was also found in dorsal root ganglia and genital ridge 4.Although the function of GlyR α4 at its sites of expression is still unknown, homomeric α4 receptors are able to form fully functional glycine-gated Cl- channels in vitro that can be antagonized with strychnine5.

Alternative names: Glycine Receptor alpha4 , Glycine receptor subunit alpha-4, GLRA4

Gene ID: 14657

NCBI Accession: NR_164162

UniProt: Q61603