MC2R antibody (Extracellular)

Catalog No. ABIN7043322

Product Details anti-MC2R Antibody

Target: MC2R (Melanocortin 2 Receptor (Adrenocorticotropic Hormone) (MC2R))

Binding Specificity: AA 87-99, Extracellular

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MC2R antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Purpose: A Rabbit Polyclonal Antibody to Melanocortin Receptor 2

Specificity: 1st extracellular loop

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat,mouse - identical

Characteristics: Anti-MC2 Receptor (extracellular) Antibody is directed against the first extracellular loop of the human melanocortin receptor 2. Anti-MC2 Receptor (extracellular) Antibody (ABIN7043322 and ABIN7044594)) can be used in western blot and immunohistochemistry applications. It has been designed to recognize MC2R from human, mouse, and rat samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RNMGYLKPRGSFE, corresponding to amino acid residues 87-99 of human MC2R

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100

Application Dilutions Western blot wb: 1:400

Comment:

Negative Control: BLP-MR022

Blocking Peptide: BLP-MR022

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for MC2R

Target: MC2R (Melanocortin 2 Receptor (Adrenocorticotropic Hormone) (MC2R))

Alternative Name: MC2R (MC2R Products)

Background: Melanocortin receptor 2, MC2R, MC2-R, Adrenocorticotropic hormone receptor, ACTH receptor, ACTHR, ACTH-R, Adrenocorticotropin receptor,Melanocortin receptor 2 (MC2R) belongs to a five-member receptor family known as the melanocortin receptors. The melanocortin receptors are members of the 7 transmembrane domain, G-protein coupled receptor (GPCR) superfamily.The receptors' ligands, the melanocortins, are a group of structurally derived peptides consisting of α-, β- and γ melanocyte stimulating hormone (α, β, γ-MSH) and the adrenocorticotropic hormone (ACTH), all of which are derived from the post-translational processing of a common precursor peptide, proopiomelanocortin (POMC).1,2,3One of the most salient features of the melanocortin signaling system is the presence of two endogenous antagonists, proteins that bind specifically to the receptor but instead of activating it have an inhibitory effect. The antagonist proteins are termed agouti (or agouti signaling protein, ASP) and agouti-related protein (AGRP).4All five melanocortin receptors bind their agonists (the melanocortins) and their endogenous antagonists (agouti and AGRP) with different affinities.MC2R can also be described as the ACTH receptor as it is exclusively activated by the ACTH hormone. In fact, MC2R mediates all the physiological effects of ACTH in the adrenal cortex, that is, the regulation and production of steroids, their circadian variation and their stress-related fluctuations.1,2,3 In addition, mutations that result in MC2R functional impairment are associated with familial glucocorticoid deficiency.4The receptor transduces signals via Gs resulting in the activation of adenylate cyclase and production of cAMP.

Alternative names: MC2 Receptor, Melanocortin receptor 2, MC2R, MC2-R, Adrenocorticotropic hormone receptor, ACTH receptor, ACTHR, ACTH-R, Adrenocorticotropin receptor

Gene ID: 4158

NCBI Accession: NM_000529

UniProt: Q01718

Pathways: cAMP Metabolic Process