NFASC antibody (Intracellular)

Catalog No. ABIN7043360

Product Details anti-NFASC Antibody

Target: NFASC (Neurofascin (NFASC))

Binding Specificity: AA 1142-1155, Intracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFASC antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Purpose: A Rabbit Polyclonal Antibody to Neurofascin

Specificity: Intracellular, C-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,human - identical

Characteristics: Anti-Neurofascin Antibody (ABIN7043360, ABIN7044538 and ABIN7044539)) is a highly selective antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize NFASC from human, mouse, and rat samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)REKKDVPLGPEDPK, corresponding to amino acid residues 1142-1155 of rat NFASC

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:800

Comment:

Negative Control: BLP-IP025

Blocking Peptide: BLP-IP025

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for NFASC

Target: NFASC (Neurofascin (NFASC))

Alternative Name: NFASC (NFASC Products)

Background: NFASC, NRCAML, NF,Neurofascin belongs to the L1 subgroup of the immunoglobulin superfamily of cell adhesion molecules and is an axon associated surface glycoprotein implicated in axonal growth and fasciculation.Neurofascin is comprised of four structural elements: At the NH2 terminus, neurofascin contains six Ig-like motifs of the C2 subcategory followed by four fibronectin type III (FNIII)-related repeats. Between the FNIII-like repeats and the plasma membrane spanning region, neurofascin contains a 75-amino acid residues rich in proline, alanine and threonine which might be the target of extensive O-linked glycosylation. A transmembrane segment is followed by a 113-amino acid residues-long cytoplasmic domain1.Neurofascin associates with sodium channel β1 (NaVβ1) units via interaction of its first Ig-like domain and second fibronectin type III-like domain with the extracellular Ig-like domain of β1. NaVβ1 subunits localize to nodes of Ranvier with neurofascin in sciatic nerve axons. NaVβ1 and neurofascin are associated as early as postnatal day 5, during the period that nodes of Ranvier are in formation2.Unlike L1 and other subgroup members, neurofascin is subjected to extensive alternative splicing that is regulated during embryonic development of the brain. An oligodendroglial isoform of neurofascin, NF155, serves as a glial receptor for the paranodin/Caspr-contactin complex. The extracellular domain of NF155 binds to the complex at the cell surface3.

Alternative names: Neurofascin, NFASC, NRCAML, NF

Gene ID: 116690

NCBI Accession: NM_001005388

UniProt: P97685

Pathways: Cell-Cell Junction Organization