Peroxisomal Biogenesis Factor 5-Like (PEX5L) (AA 151-165), (Intracellular) antibody
Quick Overview for Peroxisomal Biogenesis Factor 5-Like (PEX5L) (AA 151-165), (Intracellular) antibody (ABIN7887014)
Target
See all Peroxisomal Biogenesis Factor 5-Like (PEX5L) AntibodiesReactivity
Host
Clonality
Conjugate
Application
Grade
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Binding Specificity
- AA 151-165, Intracellular
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Purpose
- A Rabbit Polyclonal Antibody to PEX5L (TRIP8b)
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Cross-Reactivity
- Mouse, Rat
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Predicted Reactivity
- rabbit - 14, human - 13,15 amino acid residues identical,Mouse - identical
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Characteristics
- Anti-PEX5L (TRIP8b) Antibody is directed against an epitope of the rat PEX5-related protein. Anti-PEX5L (TRIP8b) Antibody (ABIN7043433, ABIN7045126 and ABIN7045127) can be used in western blot and immunohistochemistry applications. It has been designed to recognize PEX5Rp from rat, mouse and human samples.
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Purification
- Affinity purified on immobilized antigen.
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Immunogen
- (C)EKWDDVKFHGDRTSK, corresponding to amino acid residues 151-165 of rat PEX5-related protein
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Isotype
- IgG
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Application Notes
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WB: 1:400
FC: The optimal concentration should be determined by the user
ICC: The optimal concentration should be determined by the user
IHC: 1:300
IP: The optimal concentration should be determined by the user
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Comment
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Negative Control: (ABIN7236249)
Blocking Peptide: (ABIN7236249)
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.
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Concentration
- 0.8 mg/mL
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Buffer
- PBS pH 7.4, 1 % BSA with 0.05 % sodium azide
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- -20 °C
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Storage Comment
- The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C
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- Peroxisomal Biogenesis Factor 5-Like (PEX5L)
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Alternative Name
- PEX5L
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Background
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Synonyms: PEX5R, PEX5Rp, PEX5-related protein, PEX5-like protein, Peroxin-5-related protein, PEX2-related protein, PXR2, Peroxisome biogenesis factor 5-like, TPR-containing Rab8b-interacting protein
Description: PEX5R (also called TRIP8b, tetratricopetide repeat-containing Rab8b-interacting protein) is a cytoplasmic protein expressed as a family of alternatively spliced isoforms. PEX5R /TRIP8b isoforms contain a large constant domain preceded by a variable region. The C-terminal half of TRIP8b comprises a tetratricopeptide repeat (TPR) protein binding domain1. Analysis of the Pex5p-like protein revealed the presence of a tetratricopeptide repeat (TPR) domain in its C-terminal half consisting of seven TPR motifs. Pex5R is almost exclusively expressed in brain2.Recent studies provide strong evidence that PEX5R interacts with the carboxyl-terminal region of Hyperpolarization-activated cyclic nucleotide-gated (HCN) channels and regulates their cell-surface expression level and cyclic nucleotide dependence3,4. HCN channels are key modulators of neuronal activity by providing the depolarizing cation current involved in rhythmogenesis, dendritic integration, and synaptic transmission. In contrast, Gosh et al. have shown that human PEX5R can bind to peroxisomal targeting signal-1 (PTS1) ligands5.Furthermore it was shown that enzyme alanine-glyoxylate aminotransferase (AGT) is recognized by PEX5R in the cytoplasm, which allows its subsequent translocation into the peroxisome6. TRIP8b may play a role in both normal neuronal function and in aberrant neuronal excitability associated with neurological diseases7.
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Gene ID
- 286937
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NCBI Accession
- NM_016559
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UniProt
- Q925N3
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Pathways
- Positive Regulation of Peptide Hormone Secretion, Maintenance of Protein Location
Target
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