Peroxisomal Biogenesis Factor 5-Like (PEX5L) (AA 151-165) antibody

Catalog No. ABIN7043433

Product Details

Target: Peroxisomal Biogenesis Factor 5-Like (PEX5L)

Binding Specificity: AA 151-165

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF)

Purpose: A Rabbit Polyclonal Antibody to PEX5L (TRIP8b)

Specificity: Intracelular.

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: rabbit - 14, human - 13,15 amino acid residues identical,Mouse - identical

Characteristics: Anti-PEX5L (TRIP8b) Antibody is directed against an epitope of the rat PEX5-related protein. Anti-PEX5L (TRIP8b) Antibody (ABIN7043433, ABIN7045126 and ABIN7045127) can be used in western blot and immunohistochemistry applications. It has been designed to recognize PEX5Rp from rat, mouse and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)EKWDDVKFHGDRTSK, corresponding to amino acid residues 151-165 of rat PEX5-related protein

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:400

Comment:

Negative Control: (ABIN7236249)

Blocking Peptide: (ABIN7236249)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details

Target: Peroxisomal Biogenesis Factor 5-Like (PEX5L)

Alternative Name: PEX5L (PEX5L Products)

Background: PEX5R, PEX5Rp, PEX5-related protein, PEX5-like protein, Peroxin-5-related protein, PEX2-related protein, PXR2, Peroxisome biogenesis factor 5-like, TPR-containing Rab8b-interacting protein,PEX5R (also called TRIP8b, tetratricopetide repeat-containing Rab8b-interacting protein) is a cytoplasmic protein expressed as a family of alternatively spliced isoforms. PEX5R /TRIP8b isoforms contain a large constant domain preceded by a variable region. The C-terminal half of TRIP8b comprises a tetratricopeptide repeat (TPR) protein binding domain1. Analysis of the Pex5p-like protein revealed the presence of a tetratricopeptide repeat (TPR) domain in its C-terminal half consisting of seven TPR motifs. Pex5R is almost exclusively expressed in brain2.Recent studies provide strong evidence that PEX5R interacts with the carboxyl-terminal region of Hyperpolarization-activated cyclic nucleotide-gated (HCN) channels and regulates their cell-surface expression level and cyclic nucleotide dependence3,4. HCN channels are key modulators of neuronal activity by providing the depolarizing cation current involved in rhythmogenesis, dendritic integration, and synaptic transmission. In contrast, Gosh et al. have shown that human PEX5R can bind to peroxisomal targeting signal-1 (PTS1) ligands5.Furthermore it was shown that enzyme alanine-glyoxylate aminotransferase (AGT) is recognized by PEX5R in the cytoplasm, which allows its subsequent translocation into the peroxisome6. TRIP8b may play a role in both normal neuronal function and in aberrant neuronal excitability associated with neurological diseases7.

Alternative names: PEX5L (TRIP8b), PEX5R, PEX5Rp, PEX5-related protein, PEX5-like protein, Peroxin-5-related protein, PEX2-related protein, PXR2, Peroxisome biogenesis factor 5-like, TPR-containing Rab8b-interacting protein

Gene ID: 286937

NCBI Accession: NM_016559

UniProt: Q925N3

Pathways: Positive Regulation of Peptide Hormone Secretion, Maintenance of Protein Location