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Peroxisomal Biogenesis Factor 5-Like (PEX5L) (AA 151-165) antibody

PEX5L Reactivity: Rat IHC, WB, IF Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN7043433
  • Target See all Peroxisomal Biogenesis Factor 5-Like (PEX5L) Antibodies
    Peroxisomal Biogenesis Factor 5-Like (PEX5L)
    Binding Specificity
    • 2
    • 1
    • 1
    AA 151-165
    Reactivity
    • 4
    • 1
    • 1
    • 1
    Rat
    Host
    • 6
    • 1
    Rabbit
    Clonality
    • 6
    • 1
    Polyclonal
    Conjugate
    • 7
    Un-conjugated
    Application
    • 7
    • 7
    • 4
    • 1
    Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF)
    Purpose
    A Rabbit Polyclonal Antibody to PEX5L (TRIP8b)
    Specificity
    Intracelular.
    Cross-Reactivity
    Mouse, Rat
    Predicted Reactivity
    rabbit - 14, human - 13,15 amino acid residues identical,Mouse - identical
    Characteristics
    Anti-PEX5L (TRIP8b) Antibody is directed against an epitope of the rat PEX5-related protein. Anti-PEX5L (TRIP8b) Antibody (ABIN7043433, ABIN7045126 and ABIN7045127)) can be used in western blot and immunohistochemistry applications. It has been designed to recognize PEX5Rp from rat, mouse and human samples.
    Purification
    Affinity purified on immobilized antigen.
    Immunogen

    Immunogen: Synthetic peptide

    Immunogen Sequence: (C)EKWDDVKFHGDRTSK, corresponding to amino acid residues 151-165 of rat PEX5-related protein

    Isotype
    IgG
  • Application Notes

    Antigen preadsorption control: 1 μg peptide per 1 μg antibody

    Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

    Application Dilutions Western blot wb: 1:400

    Comment

    Negative Control: BLP-PR070

    Blocking Peptide: BLP-PR070

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    0.2 mL double distilled water (DDW).
    Concentration
    1 mg/mL
    Buffer
    PBS pH 7.4
    Storage
    4 °C,-20 °C
    Storage Comment

    Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

    Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

  • Target
    Peroxisomal Biogenesis Factor 5-Like (PEX5L)
    Alternative Name
    PEX5L (PEX5L Products)
    Background
    PEX5R, PEX5Rp, PEX5-related protein, PEX5-like protein, Peroxin-5-related protein, PEX2-related protein, PXR2, Peroxisome biogenesis factor 5-like, TPR-containing Rab8b-interacting protein,PEX5R (also called TRIP8b, tetratricopetide repeat-containing Rab8b-interacting protein) is a cytoplasmic protein expressed as a family of alternatively spliced isoforms. PEX5R /TRIP8b isoforms contain a large constant domain preceded by a variable region. The C-terminal half of TRIP8b comprises a tetratricopeptide repeat (TPR) protein binding domain1. Analysis of the Pex5p-like protein revealed the presence of a tetratricopeptide repeat (TPR) domain in its C-terminal half consisting of seven TPR motifs. Pex5R is almost exclusively expressed in brain2.Recent studies provide strong evidence that PEX5R interacts with the carboxyl-terminal region of Hyperpolarization-activated cyclic nucleotide-gated (HCN) channels and regulates their cell-surface expression level and cyclic nucleotide dependence3,4. HCN channels are key modulators of neuronal activity by providing the depolarizing cation current involved in rhythmogenesis, dendritic integration, and synaptic transmission. In contrast, Gosh et al. have shown that human PEX5R can bind to peroxisomal targeting signal-1 (PTS1) ligands5.Furthermore it was shown that enzyme alanine-glyoxylate aminotransferase (AGT) is recognized by PEX5R in the cytoplasm, which allows its subsequent translocation into the peroxisome6. TRIP8b may play a role in both normal neuronal function and in aberrant neuronal excitability associated with neurological diseases7.

    Alternative names: PEX5L (TRIP8b), PEX5R, PEX5Rp, PEX5-related protein, PEX5-like protein, Peroxin-5-related protein, PEX2-related protein, PXR2, Peroxisome biogenesis factor 5-like, TPR-containing Rab8b-interacting protein
    Gene ID
    286937
    NCBI Accession
    NM_016559
    UniProt
    Q925N3
    Pathways
    Positive Regulation of Peptide Hormone Secretion, Maintenance of Protein Location
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