KCNK12 antibody (Extracellular)

Catalog No. ABIN7043449

Product Details anti-KCNK12 Antibody

Target: KCNK12 (Potassium Channel, Subfamily K, Member 12 (KCNK12))

Binding Specificity: AA 61-73, Extracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KCNK12 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purpose: A Rabbit Polyclonal Antibody to KCNK12 Channel

Specificity: 1st extracellular loop

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,human - identical

Characteristics: Anti-KCNK12 (THIK-2) (extracellular) Antibody (ABIN7043449, ABIN7045070 and ABIN7045071)) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize KCNK12 from rat and mouse and human samples.

Purification: Affinity purified on immobilized antigen.

Grade: KO Validated

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)ESPGEAEARARWG, corresponding to amino acid residues 61-73 of rat KCNK12

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:400

Application Dilutions Western blot wb: 1:200-1:400

Comment:

Negative Control: BLP-PC169

Blocking Peptide: BLP-PC169

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for KCNK12

Target: KCNK12 (Potassium Channel, Subfamily K, Member 12 (KCNK12))

Alternative Name: KCNK12 (KCNK12 Products)

Background: Potassium channel subfamily K member 12, Tandem pore domain halothane-inhibited K+ channel 2, K2P12.1,Potassium channels form the largest ion channel family with over 70 genes identified in humans. 2-pore domain channels (K2P) are believed to be responsible for background or leak K+ currents and they are widely expressed in the nervous system. They are involved in resting potential control and firing pattern of excitable cells1. The K2P channel family is comprised of at least 16 members.The KCNK12 channel (THIK-2, K2P12.1) has two pore domains (M1 and P1) and a long M1-P1 linker. It has a long cytosolic C terminus and a very unusual N-terminal domain containing many cysteine, proline and arginine residues. This N-terminal domain cannot be found in the similar THIK-1 channel and is highly conserved in mammals.Until recently, the THIK-2 channel was considered to be a "silent" channel and could not be functionally expressed in mammalian and non-mammalian cell lines. When removing the N-terminal both THIK-1 and THIK-2 channels exhibit similar current-voltage relations. In addition, both channels have 64 % identical amino-acids in humans and 59 % in rats. Only a small fraction of THIK-2 channel is localized to the cell membrane surface and this can be increased by deleting the original retention/retrieval signal RRR in its N-terminus.The THIK-2 channel is highly expressed in the cortex, cerebellum hippocampus, thalamus, inferior colliculi of the midbrain and the olfactory bulb. It is also expressed in non-neuronal tissues such as the lungs, liver, kidney and spleen2.

Alternative names: KCNK12 (THIK-2), Potassium channel subfamily K member 12, Tandem pore domain halothane-inhibited K+ channel 2, K2P12.1

Gene ID: 64119

NCBI Accession: NM_022055

UniProt: Q9ERS1