KCNK16 antibody (Extracellular)

Catalog No. ABIN7043450

Product Details anti-KCNK16 Antibody

Target: KCNK16 (Potassium Channel, Subfamily K, Member 16 (KCNK16))

Binding Specificity: AA 75-88, Extracellular

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KCNK16 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Purpose: A Rabbit Polyclonal Antibody to KCNK16 Channel

Specificity: 1st extracellular loop

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat,mouse - identical

Characteristics: Anti-KCNK16 (TALK-1) (extracellular) Antibody (ABIN7043450, ABIN7045072 and ABIN7045073)) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, analysis. The antibody recognizes an extracellular epitope, and can potentially be used for detecting the protein in living cells. It has been designed to recognize TALK-1 from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Grade: KO Validated

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)EAWVKGVNPKGNST, corresponding to amino acid residues 75-88 of human KCNK16 

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:150

Application Dilutions Western blot wb: 1:600

Comment:

Negative Control: BLP-PC170

Blocking Peptide: BLP-PC170

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for KCNK16

Target: KCNK16 (Potassium Channel, Subfamily K, Member 16 (KCNK16))

Alternative Name: KCNK16 (KCNK16 Products)

Background: Potassium two pore domain channel subfamily K member 16, TWIK-related alkaline pH -activated K+ channel 1, K2P16.1,The KCNK16 gene encodes the TALK-1 channel, an abundant K+ channel transcript found in mouse and in human β-cells. There are four human TALK-1 transcript variants, including two that form functional K+ channels (TALK-1a (transcript variant 2) and TALK1-b (transcript variant 3)1,2. The TALK-1 structure includes 4 transmembrane domains, 2 extracellular loops, and intracellular N- and C-termini. The C-terminal tail of TALK-1 is a critical component that influences the magnitude of TALK-1 channel activation, while the N-terminus is important for protein interaction1,2.TALK-1, two-pore domain K+ (K2P16.1) channel is responsible for modulating glucose stimulated insulin secretion that relies on β-cell Ca2+ influx. The channel produces outwardly rectifying, non-inactivating K+ currents, which are enhanced by elevations in extracellular pH . TALK-1 is thus pH -sensitive, with increased activity under alkaline conditions. In addition, it can be activated by singlet oxygen and nitric oxide.A gain-of-function polymorphism in the KCNK16 gene is associated with increased risk for developing type-2 diabetes1,2.

Alternative names: KCNK16 (TALK-1), Potassium two pore domain channel subfamily K member 16, TWIK-related alkaline pH -activated K+ channel 1, K2P16.1

Gene ID: 83795

NCBI Accession: NM_032115

UniProt: Q96T55