KCNK2 antibody (Intracellular)

Catalog No. ABIN7043452

Product Details anti-KCNK2 Antibody

Target: KCNK2 (Potassium Channel, Subfamily K, Member 2 (KCNK2))

Binding Specificity: AA 8-25, Intracellular

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KCNK2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC)

Purpose: A Rabbit Polyclonal Antibody to KCNK2 Channel

Specificity: Intracellular, N-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat - identical, mouse - 17,18 amino acid residues identical

Characteristics: Anti-KCNK2 (TREK-1) Antibody (ABIN7043452, ABIN7044957 and ABIN7044958)) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize TREK-1 from mouse, rat, and human samples.

Purification: Affinity purified on immobilized antigen.

Grade: KO Validated

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: DPKSAAQNSKPRLSFSTK(C), corresponding to residues 8-25 of human KCNK2 

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IP|IHC|ICC

Negative Control: BLP-PC047

Blocking Peptide: BLP-PC047

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for KCNK2

Target: KCNK2 (Potassium Channel, Subfamily K, Member 2 (KCNK2))

Alternative Name: KCNK2 (KCNK2 Products)

Background: Potassium channel subfamily K member 2, Outward rectifying potassium channel protein TREK-1, Twik-related K+ channel 1, K2P2.1, TPKC1,KCNK2 (also named TWIK-related K+ channel, TREK-1 or K2P2.1) is a member of the 2-pore (2P) domain K+ channels family that includes at least 16 members. These channels show little time- or voltage-dependence and are considered to be "leak" or "background" K+ channels, thereby generating background currents which help set the membrane resting potential and cell excitation.The K2P channels have a signature topology that includes four transmembrane domains and two pore domains with intracellular N- and C- termini. K2P channels are regulated by diverse physical and chemical stimuli including temperature, changes in intracellular pH , mechanical stretch, inhalation anesthetics, etc. The channels can then be subclassified based in their specific activators. KCNK2 can be integrated to a K2P subfamily that includes K2P4.1 (TRAAK) and K2P10.1 (TREK2) that are activated by intracellular unsaturated fatty acids such as arachidonic acid, lysophosphatidic acid and mechanical stretch. In addition, KCNK2 can also be activated by general anesthetics such as halothane and chloroform and intracellular acidification.KCNK2 expression in humans is largely restricted to the brain with some expression in ovary and small intestine while KCNK2 expression in rodents is more widespread.KCNK2 has an important role in mood regulation, as knockout mice show resistance to depression, suggesting that KCNK2 may be a potential target for anti-depressants.KCNK2 is involved in the fast release of glutamate from astrocytes. It requires the activation of Gαi, dissociation of Gβγ, followed by the opening of the glutamate permeable KCNK2 (TREK-1) K+ channel through its interaction with Gβγ. For this purpose, KCNK2 is localized at the cell surface of the cell body and processes of astrocytes.

Alternative names: KCNK2 (TREK-1), Potassium channel subfamily K member 2, Outward rectifying potassium channel protein TREK-1, Twik-related K+ channel 1, K2P2.1, TPKC1

Gene ID: 3776

NCBI Accession: NM_014217

UniProt: O95069