KCNK5 antibody (Intracellular)

Catalog No. ABIN7043455

Product Details anti-KCNK5 Antibody

Target: KCNK5 (Potassium Channel, Subfamily K, Member 5 (KCNK5))

Binding Specificity: AA 483-499, Intracellular

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KCNK5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purpose: A Rabbit Polyclonal Antibody to KCNK5 Channel

Specificity: Intracellular, C-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse - 14,17 amino acid residues identical

Characteristics: Anti-KCNK5 (TASK-2) Antibody is directed against an epitope of human KCNK5. Anti-KCNK5 (TASK-2) Antibody (ABIN7043455 and ABIN7044945)) can be used in western blot and immunohistochemistry applications. It has been designed to recognize KCNK5 from rat, mouse and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)YEQLMNEYNKANSPKGT, amino acid residues 483-499 of human KCNK5 

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:50

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: ICC

Negative Control: BLP-PC037

Blocking Peptide: BLP-PC037

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for KCNK5

Target: KCNK5 (Potassium Channel, Subfamily K, Member 5 (KCNK5))

Alternative Name: KCNK5 (KCNK5 Products)

Background: Potassium channel subfamily K member 5, K2P5.1, TWIK-related acid sensitive K+ channel 2,KCNK5 (also named TWIK-related acid sensitive K+ channel 2, TASK-2 or K2P5.1) is a member of the 2-pore (2P) domain K+ channels family that includes at least 16 members. These channels show little time- or voltage-dependence and are considered to be "leak" or "background" K+ channels, thereby generating background currents which help set the membrane resting potential and cell excitation.The K2P channels have a signature topology that includes four transmembrane domains and two pore domains with intracellular N- and C- termini. The functional channel is believed to be a dimer.K2P channels are regulated by diverse physical and chemical stimuli including temperature, changes in intracellular pH , mechanical stretch, inhalation anesthetics, etc. The channels can then be subclassified based in their specific activators.As indicated by its name, KCNK5 (K2P5.1) is sensitive to variations in external pH . KCNK5 current is maximal at pH 8.8 but it is only 10 % at pH 6.5. The channel is expressed in several epithelial tissues including pancreas, lung, small intestine and specially kidney. In the kidney KCNK5 has an important function in NaHCO3 absorption in the proximal tubule as knockout mice for KCNK5 display metabolic acidosis and hypotension.

Alternative names: KCNK5 (TASK-2), Potassium channel subfamily K member 5, K2P5.1, TWIK-related acid sensitive K+ channel 2

Gene ID: 8645

NCBI Accession: NM_003740

UniProt: O95279