KIR5.1 antibody (Intracellular)
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- Target See all KIR5.1 (KCNJ16) Antibodies
- KIR5.1 (KCNJ16) (Potassium Inwardly-Rectifying Channel, Subfamily J, Member 16 (KCNJ16))
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Binding Specificity
- AA 311-325, Intracellular
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Reactivity
- Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This KIR5.1 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
- Purpose
- A Rabbit Polyclonal Antibody to Kir5.1 Channel
- Specificity
- Intracellular, C-terminus
- Cross-Reactivity
- Human, Mouse, Rat
- Predicted Reactivity
- Mouse - identical, human 14,15 amino acid residues-identical
- Characteristics
- Anti-Kir5.1 Antibody (ABIN7043478, ABIN7045023 and ABIN7045024)) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Kir5.1 from rat, mouse, and human samples.
- Purification
- Affinity purified on immobilized antigen.
- Immunogen
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Immunogen: Synthetic peptide
Immunogen Sequence: HDVLEVKRKYYKVNC, corresponding to amino acid residues 311-325 of rat KCNJ16
- Isotype
- IgG
- Top Product
- Discover our top product KCNJ16 Primary Antibody
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- Application Notes
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100
Application Dilutions Western blot wb: 1:200
- Comment
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Negative Control: BLP-PC123
Blocking Peptide: BLP-PC123
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- 0.2 mL double distilled water (DDW).
- Concentration
- 1 mg/mL
- Buffer
- PBS pH 7.4
- Storage
- 4 °C,-20 °C
- Storage Comment
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- Target
- KIR5.1 (KCNJ16) (Potassium Inwardly-Rectifying Channel, Subfamily J, Member 16 (KCNJ16))
- Alternative Name
- KCNJ16 (KCNJ16 Products)
- Background
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Inward rectifier potassium channel 16, KCNJ16, BIR9,Kir5.1 is a member of the family of inward rectifying K+ channels. The family includes 15 members that are structurally and functionally different from the voltage-dependent K+ channels.The family's protein topology consists of two transmembrane domains that flank a single and highly conserved pore region with intracellular N- and C-termini. As is the case for the voltage-dependent K+ channels the functional unit for the Kir channels is composed of four subunits that can assemble as either homo- or heteromers.Kir channels are characterized by a K+ efflux that is limited by depolarizing membrane potentials thus making them essential for controlling resting membrane potential and K+ homeostasis.1Kir5.1 can co-assemble with other Kir channels such as Kir2.1, Kir4.1 and Kir4.2. In fact, heteromeric Kir4.1/Kir5.1 form the basolateral small-conductance K+ channel in distal nephron segments that is responsible for generating the basolateral membrane potential that determines the magnitude and direction of K+ diffusion from cell to peritubular fluid in the nephron.2The Kir5.1 homomeric channel was largely thought to be non-functional, although recent evidence demonstrates that Kir5.1 homomers can form functional channels when co-expressed with the anchor protein PSD-95 in the brain where it is abundantly expressed.3
Alternative names: Kir5.1, Inward rectifier potassium channel 16, KCNJ16, BIR9 - Gene ID
- 29719
- NCBI Accession
- NM_018658
- UniProt
- P52191
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