Kv2.2 antibody (Intracellular)

Catalog No. ABIN7043510

Product Details anti-Kv2.2 Antibody

Target: Kv2.2 (KCNB2) (Potassium Voltage-Gated Channel, Shab-Related Subfamily, Member 2 (KCNB2))

Binding Specificity: AA 859-873, Intracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Kv2.2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunochromatography (IC), Immunofluorescence (IF)

Purpose: A Rabbit Polyclonal Antibody to KV2.2 (KCNB2) Channel

Specificity: Intracellular, C-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse - identical, human - 13,15 amino acid residues identical

Characteristics: Anti-Kv2.2 (KCNB2) Antibody is directed against an intracellular epitope of the rat KV2.2 channel. Anti-KV2.2 (KCNB2) Antibody (ABIN7043510, ABIN7045017 and ABIN7045018)) can be used in western blot, immunoprecipitation, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize KV2.2 from rat, human, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: CRQDIYQAVGEVKKD, corresponding to amino acid residues 859-873 of rat KV2.2

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:2000

Comment:

Negative Control: BLP-PC120

Blocking Peptide: BLP-PC120

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Kv2.2

Target: Kv2.2 (KCNB2) (Potassium Voltage-Gated Channel, Shab-Related Subfamily, Member 2 (KCNB2))

Alternative Name: KCNB2 (KCNB2 Products)

Background: Voltage-gated potassium channel subfamily B member 2, CDRK,KV2.2 is a member of the voltage-gated K+ channel superfamily. Together with the closely related KV2.1 protein they form the KV2 subfamily also known as Shab.As with all KV channels, KV2.2 possesses the signature structure of the voltage-dependent K+ channels: six membrane-spanning domains with intracellular N and C termini. The functional KV channel is a tetramer that can either be a homotetramer or a heteromer of KV2.2 and KV2.1 subunits.Both KV2.2 and KV2.1 channels are known as delayed rectifiers that is, channels that are activated by changes in membrane potential (depolarization) but inactivate very slowly. The current they form is known as IK or IDR. Accessory subunits such as KChaP and the electrically silent a subunits KV8 and KV9 can modulate biochemical and biophysical properties of KV2.2. KV2.2 is expressed in the brain and in peripheral tissues such as gastrointestinal and vascular smooth muscle. Physiologically, KV2.2 has a role in maintaining membrane potential and in modulating electrical excitability in neurons. In smooth muscle cells, KV2.2 is probably involved in maintaining the contractile capacity of the cells.The pharmacology of the KV2.2 channel is somewhat limited. The channel is blocked by the classical K+ channel blockers tetraethylammonium (TEA) and 4-aminopyridine (4-AP). More recently, the small peptide toxin Stromatoxin-1 has been found to be a potent and more specific inhibitor of the KV2 subfamily.

Alternative names: KV2.2 (KCNB2), Voltage-gated potassium channel subfamily B member 2, CDRK

Gene ID: 117105

NCBI Accession: NM_004770

UniProt: Q63099