KCNAB1 antibody (AA 54-67)

Catalog No. ABIN7043511

Product Details anti-KCNAB1 Antibody

Target: KCNAB1 (Potassium Voltage-Gated Channel, Shaker-Related Subfamily, beta Member 1 (KCNAB1))

Binding Specificity: AA 54-67

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KCNAB1 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: A Rabbit Polyclonal Antibody to KVβ1 Subunit

Specificity: N-terminus

Cross-Reactivity: Human, Mouse, Rat

Cross-Reactivity (Details): This antibody will recognize the "canonical" splice isoform of human KVβ1 (also known as KVβ1.3). It will not recognize splice variants KVβ1.1 and KVβ1.2. For more information see human KVβ1 entry Q14722.

Predicted Reactivity: Mouse,human - identical

Characteristics: Anti-Kvbeta1 Antibody is directed against an epitope of rat KVβ1. Anti-KVβ1 Antibody (ABIN7043511 and ABIN7045031)) can be used in western blot analysis. This antibody will recognize the splice isoform of human KVβ1 (also known as KVβ1.1). It won't recognize splice variants KVβ1.2 and KVβ1.3. For more information, see human KVβ1 entry Q14722 and Q14722-2. It has been designed to recognize KVβ1 from rat, mouse and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)TPQHHISLKESTAK, corresponding to amino acid residues 54-67 of rat KCNAB1 

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: BLP-PC127

Blocking Peptide: BLP-PC127

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for KCNAB1

Target: KCNAB1 (Potassium Voltage-Gated Channel, Shaker-Related Subfamily, beta Member 1 (KCNAB1))

Alternative Name: KCNAB1 (KCNAB1 Products)

Background: Voltage-gated potassium channel subunit beta-1, KV beta 1, KVb1, KCNAB1,Voltage-gated K+ channels represent a structurally and functionally diverse group of membrane proteins. These channels establish the resting membrane potential and modulate the frequency and duration of action potentials in nerve and muscle1. The duration of the open states of K+ channels is determined by the rates of deactivation and inactivation. KV channels are hetero-oligomeric complexes consisting of two different types of subunits: membrane-bound, pore-forming α subunits and the smaller β subunits. The β subunits appear to be peripheral proteins tightly associated with the cytoplasmic side of the α subunits2. The inactivation gate can be formed by an N-terminal segment of an auxiliary β-subunit, as in the complex of KVβ1 and KVα family channels3. There are three mammalian KVβ genes: KVβ1, KVβ2 and KVβ3. Additional variability in the KVβ1 family results from alternative splicing in the amino terminal region, thus yielding the KVβ1.1, KVβ1.2, and KVβ1.3 subunits4.

Alternative names: KVbeta1, Voltage-gated potassium channel subunit beta-1, KV beta 1, KVb1, KCNAB1

Gene ID: 29737

NCBI Accession: NM_003471

UniProt: P63144