KCND1 antibody (Intracellular)

Catalog No. ABIN7043532

Product Details anti-KCND1 Antibody

Target: KCND1 (Kcnd1) (Potassium Voltage-Gated Channel, Shal-Related Subfamily, Member 1 (Kcnd1))

Binding Specificity: AA 538-550, Intracellular

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KCND1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC)

Purpose: A Rabbit Polyclonal Antibody to KV4.1 Channel

Specificity: Intracellular, C-terminal domain

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,rat - 12,13 amino acid residues identical

Characteristics: Anti-Kv4.1 (KCND1) Antibody is directed against an intracellular epitope of human KV4.1. The Anti-KV4.1 (KCND1) Antibody (ABIN7043532, ABIN7045015 and ABIN7045016)) can be used for western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize KV4.1 from rat, human, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Grade: KO Validated

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KRRAIRLANSTAS, corresponding to amino acid residues 538-550 of human KCND1 

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:400

Comment:

Cited Application: ICC

Negative Control: BLP-PC119

Blocking Peptide: BLP-PC119

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for KCND1

Target: KCND1 (Kcnd1) (Potassium Voltage-Gated Channel, Shal-Related Subfamily, Member 1 (Kcnd1))

Alternative Name: KCND1 (Kcnd1 Products)

Background: Voltage-gated potassium channel subfamily D member 1,KV4.1 is a voltage-dependent K+ channel that belongs to the Shal channel subfamily and includes two other members: KV4.2 and KV4.3.KV4.1 possesses the signature structure of the voltage-dependent K+ channels: six membrane-spanning domains with intracellular N- and C-termini. As with other members of the voltage-gated K+ channel superfamily, the functional channel is a tetramer that can be composed of more than one member of the Shal subfamily, i.e. heterotetramers of KV4.1 and KV4.2.The KV4 channels are characterized by activation at subthreshold membrane potentials, inactivate rapidly and recover from inactivation quickly compared with other voltage-dependent K+ channels. This type of current is known as transient A-type K+ currents. For example, depolarization-activated K+ currents in rat neostriatal cholinergic interneurons are predominantly of the A-type and attributable to coexpression of KV4.1 and KV4.2 subunits. The biophysical properties of the KV4.1 subunit can be modified by its association with auxiliary b subunits such as KChIP1 that increase KV4.1 current densities and accelerates both the inactivation and the recovery time.KV4.1 is highly expressed in the brain but is also expressed in peripheral tissues such as the colon, heart and lung.The pharmacology of KV4.1 has been less well studied than that of the other KV4 members. KV4.1 is sensitive to the classical K+ channel blockers, 4-aminopyridine and tetraethylammonium. Recently, HmTx1, a peptide blocker derived from the venom of the African tarantula Heteroscodra maculata, has been shown to be a potent KV4.1 inhibitor.

Alternative names: KV4.1 (KCND1), Voltage-gated potassium channel subfamily D member 1

Gene ID: 3750

NCBI Accession: NM_004979

UniProt: Q9NSA2