KCND3 antibody (Intracellular)

Catalog No. ABIN7043536

Product Details anti-KCND3 Antibody

Target: KCND3 (Potassium Voltage-Gated Channel, Shal-Related Subfamily, Member 3 (KCND3))

Binding Specificity: AA 451-468, Intracellular

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KCND3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunochromatography (IC)

Purpose: A Rabbit Polyclonal Antibody to KV4.3 Channel

Specificity: Intracellular, C-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat,rabbit - identical, mouse - 16,17 amino acid residues identical

Characteristics: Anti-KV4.3 Antibody (ABIN7043536, ABIN7044918 and ABIN7044919)) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunoprecipitation, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize KV4.3 from human, rat, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)NEALELTGTPEEEHMGK, corresponding to amino acid residues 451-468 of human KV4.3

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:400

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IP|IHC|ICC

Negative Control: BLP-PC017

Blocking Peptide: BLP-PC017

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for KCND3

Target: KCND3 (Potassium Voltage-Gated Channel, Shal-Related Subfamily, Member 3 (KCND3))

Alternative Name: KCND3 (KCND3 Products)

Background: Voltage-gated K+ channel subfamily D member 3, KCND3,KV4.3 is a voltage-dependent K+ channel that belongs to the Shal channel subfamily and includes two other members: KV4.1 and KV4.2.KV4.3 possesses the signature structure of the voltage-dependent K+ channels, six membrane-spanning domains with intracellular N and C termini. As with other members of the voltage-gated K+ channel superfamily, the functional channel is a tetramer that can be composed of more than one member of the Shal subfamily, i.e. heterotetramers of KV4.1 and KV4.2.The KV4 channels are characterized by activation at subthreshold membrane potentials, rapid inactivation and fast recovery from inactivation compared with other voltage-dependent K+ channels. This type of current is known as transient A-type K+ currents.1The biophysical properties of the KV4.3 subunit can be modified by its association with auxiliary β subunits such as the KChIP family that increases current densities and accelerates both the inactivation and the recovery time.KV4.3 is highly expressed in the brain where it has a key role in shaping the action potential and firing frequency of neurons. In the heart, together with KV4.2, it underlies the fast inactivating and recovering cardiac transient outward current Ito.2,3 The channel is also expressed in smooth muscle cells of several organs such as myometrium, lung and colon where its function has not been completely elucidated.Several toxins from spider venoms are potent blockers (affecting the channels in the nanomolar range) of KV4.3 channels. Among these the most potent and selective are Phrixotoxin-1 (#STP-700) (28 nM) and Phrixotoxin-2 (#STP-710) (71 nM).4

Alternative names: KV4.3, Voltage-gated K+ channel subfamily D member 3, KCND3

Gene ID: 3752

NCBI Accession: NM_172198

UniProt: O60577