KCNH4 antibody (Intracellular)

Catalog No. ABIN7043547

Product Details anti-KCNH4 Antibody

Target: KCNH4 (Potassium Voltage-Gated Channel, Subfamily H (Eag-Related), Member 4 (KCNH4))

Binding Specificity: AA 881-895, Intracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KCNH4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Purpose: A Rabbit Polyclonal Antibody to KV12.3 Channel

Specificity: Intracellular, C-terminal domain

Cross-Reactivity: Human, Rat

Predicted Reactivity: Human - identical

Characteristics: Anti-Kv12.3 (KCNH4) Antibody is directed against an epitope located at the intracellular C-terminal end of the rat KV12.3. Anti-KV12.3 (KCNH4) Antibody (ABIN7043547 and ABIN7045013)) can be used in western blot and immunohistochemical applications, and recognizes KV12.3 from rat and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: CRLNQEISRLNQEVS, corresponding to amino acids 881-895 of rat KCNH4 

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IFC

Negative Control: BLP-PC116

Blocking Peptide: BLP-PC116

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for KCNH4

Target: KCNH4 (Potassium Voltage-Gated Channel, Subfamily H (Eag-Related), Member 4 (KCNH4))

Alternative Name: KCNH4 (KCNH4 Products)

Background: Potassium voltage-gated channel subfamily H member 4, Ether-a-go-go-like potassium channel 1, ELK channel 1, rElk1, Elk3, Brain-specific eag-like channel 2, BEC2,KV12.3 (also known as KCNH4, Elk3) is a voltage-gated K+ channel that belongs to the ether-a-go-go (EAG) family. The EAG family of voltage-gated K+ channels can be subdivided into three distinct groups based on sequence homology. These are the eag (KV10) with two members, the eag-related channels (erg or KV11) and the eag-like K+ channels (elk or KV12) with three members each.As with all members of the family, KV12.3 possesses the signature structure of the voltage-dependent K+ channels: six membrane-spanning domains with intracellular N- and C- termini. The functional channel is a tetramer composed of four subunits. It has been suggested that the KV12 subfamily members can form functional heteromultimers within the subfamily.1,2KV12.3 channel distribution appears to be mainly confined to the central nervous system with some expression in peripheral organs such as the testis and lung.3The physiological function of the KV12.3 channel is yet to be established, although a role in the modulation of overall excitability of neurons has been suggested.3 Currently, there isn't much pharmacological data concerning the modulation of the KV12.3 channel except for blocking of the channel by Ba2+.3

Alternative names: KV12.3 (KCNH4), Potassium voltage-gated channel subfamily H member 4, Ether-a-go-go-like potassium channel 1, ELK channel 1, rElk1, Elk3, Brain-specific eag-like channel 2, BEC2

Gene ID: 114032

NCBI Accession: NM_012285

UniProt: Q9R1T9