KCNH5 antibody (Intracellular)

Catalog No. ABIN7043548

Product Details anti-KCNH5 Antibody

Target: KCNH5 (Potassium Voltage-Gated Channel, Subfamily H (Eag-Related), Member 5 (KCNH5))

Binding Specificity: AA 842-860, Intracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KCNH5 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF)

Purpose: A Rabbit Polyclonal Antibody to KV10.2 Channel

Specificity: Intracellular, C-terminus

Cross-Reactivity: Human, Rat

Cross-Reactivity (Details): The antibody is specific for KV10.2 and will not cross-react with KV10.1.

Predicted Reactivity: Human - 18,19 amino acid residues identical

Characteristics: Anti-KCNH5 (EAG-2) Antibody is directed against an intracellular epitope of the rat KV10.2 channel. Anti-KCNH5 (EAG-2) Antibody (ABIN7043548, ABIN7044965 and ABIN7044966)) can be used for western blot and immunohistochemistry applications. It has been designed to recognize KV10.2 from rat and human samples. The antibody is specific for KV10.2 and will not cross-react with KV10.1.

Purification: Affinity purified on immobilized antigen.

Grade: KO Validated

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)EDPKGSDSENSVTKNPLRK, corresponding to amino acid residues 842-860 of rat KCNH5 

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IP

Negative Control: BLP-PC053

Blocking Peptide: BLP-PC053

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for KCNH5

Target: KCNH5 (Potassium Voltage-Gated Channel, Subfamily H (Eag-Related), Member 5 (KCNH5))

Alternative Name: KCNH5 (KCNH5 Products)

Background: KV10.2, Ether-a-go-go potassium channel 2, hEAG2,KV10.2 is a member of the Ether-a-go-go family of voltage-activated potassium channels. To date, the family includes eight members: the closely related KV10.1 protein, the KV12 (elk) (elk1, elk2, elk3) and the KV11 (erg) proteins (erg1, erg2, erg3). The eag subfamily current is characterized by outward rectification without inactivation and slow activation kinetics. No specific pharmacological blocker has been described.Tissue distribution of KV10.2 expression in rats is restricted to neuronal tissue with greater expression in the cortex and thalamus. In humans, KV10.2 mRNA was detected in heart and skeletal muscle in addition to brain. The physiological role of KV10.2 is currently unknown.

Alternative names: KCNH5 (EAG-2), KV10.2, Ether-a-go-go potassium channel 2, hEAG2

Gene ID: 171146

NCBI Accession: NM_139318

UniProt: Q9EPI9