PTGFR antibody (AA 233-246)

Catalog No. ABIN7043565

Product Details anti-PTGFR Antibody

Target: PTGFR (Prostaglandin F Receptor (FP) (PTGFR))

Binding Specificity: AA 233-246

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PTGFR antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purpose: A Rabbit Polyclonal Antibody to Prostaglandin F2-α Receptor

Specificity: 3rd intracellular loop

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse - identical, human - 13,14 amino acid residues identical

Characteristics: Anti-Prostaglandin F2-α Receptor/PTGFR Antibody (ABIN7043565, ABIN7045120 and ABIN7045121)) is a highly selective antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize PTGFR from human, mouse, and rat samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RSQQHRQGRSHHLE, corresponding to amino acid residues 233 - 246 of rat Prostaglandin F2-alpha Receptor

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: BLP-PR067

Blocking Peptide: BLP-PR067

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for PTGFR

Target: PTGFR (Prostaglandin F Receptor (FP) (PTGFR))

Alternative Name: PTGFR (PTGFR Products)

Background: Prostaglandin F2-alpha receptor, Prostaglandin F receptor, Prostanoid FP receptor, PGF2-alpha receptor,Prostaglandins (PGs) are key lipid mediators derived from arachidonic acid that are involved in numerous physiological and pathological processes including inflammation and cardiovascular homeostasis. Each PG acts on its specific and distinct cell surface G-protein coupled receptor (GPCR) or peroxisome proliferator-activated receptor (PPAR)1.All prostaglandins are composed of a cyclopentanone nucleus with two side chains. Currently, three classes of prostaglandins are recognized, and these are categorized on the basis of the number of double bonds present within the prostaglandin molecule and on the fatty acid from which they are derived.Prostaglandin F2α (PGF2α) and its receptor (FP) are required for female reproductive functions such as luteolysis and parturition2. PGF2α was shown to be an important regulator of corpora luteal (CL) function, uterine contractility, ovulation, and embryo attachment in female swine. High affinity PGF2α receptors are present in the CL at all stages of the estrous cycle3.Apart from its pivotal role in parturition, PGF2α and its receptor are been implicated in blood pressure regulation4, atherosclerosis and other inflammation-related disorders5, cardiovascular diseases6 and bone development7. Prostaglandin F2α analogs were the first prostaglandin agonists introduced for glaucoma treatment, its mechanism of action includes reduction of intracellular pressure by increasing the uveoscleral outflow8.

Alternative names: Prostaglandin F2-alpha Receptor, PTGFR, Prostaglandin F receptor, Prostanoid FP receptor, PGF2-alpha receptor

Gene ID: 25652

NCBI Accession: NM_000959

UniProt: P43118