RAMP2 antibody (Extracellular)

Catalog No. ABIN7043596

Product Details anti-RAMP2 Antibody

Target: RAMP2 (Receptor (G Protein-Coupled) Activity Modifying Protein 2 (RAMP2))

Binding Specificity: AA 54-68, Extracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAMP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Live Cell Imaging (LCI)

Purpose: A Rabbit Polyclonal Antibody to RAMP2

Specificity: Extracellular, N-terminus

Cross-Reactivity: Mouse, Rat

Cross-Reactivity (Details): Not recommended for use with human samples.

Predicted Reactivity: Mouse - 14,15 amino acid residues identical

Characteristics: Anti-RAMP2 (extracellular) Antibody (ABIN7043596, ABIN7045221 and ABIN7045222)) is a highly selective antibody directed against an epitope of the rat protein. The antibody can be used in western blot and indirect live cell flow cytometry applications. It has been designed to recognize RAMP2 from mouse, and rat samples. It will not recognize RAMP2 from human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)HPTEDSLLSKGKMED, corresponding to amino acid residues 54-68 of rat RAMP2

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200-1:400

Comment:

Negative Control: BLP-RR022

Blocking Peptide: BLP-RR022

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for RAMP2

Target: RAMP2 (Receptor (G Protein-Coupled) Activity Modifying Protein 2 (RAMP2))

Alternative Name: RAMP2 (RAMP2 Products)

Background: Receptor activity-modifying protein 2, CRLR activity-modifying protein 2, Calcitonin receptor-like receptor activity-modifying protein 2,RAMP2, also known as receptor activity modifying protein 2, is a single-pass transmembrane protein with extracellular N-terminus and intracellular C-terminal tail. RAMP2 heterodimerizes with several G-protein coupled receptors (GPCRs) and alters their function and life cycle. RAMPs were first identified for the receptors association with calcitonin receptor-like receptor (Calcrl, CLR) as the canonical receptor for the endocrine peptide adrenomedullin (Adm, AM). In addition, RAMP2 also binds to other class B GPCRs: calcitonin receptor (Ctr), corticotrophin-releasing hormone receptor 1 (Crhr1), glucagon receptor (Gcgr), parathyroid hormone receptor 1 (Pth1r), and vasoactive intestinal polypeptide receptor types 1 and 2 (VPAC1 and VPAC2). RAMPs have an important role in receptor trafficking, including translocation from the endoplasmic reticulum to the Golgi, internalization, and recycling of the receptors1,2.The mammalian RAMP family includes three members, each encoded by single genes: RAMP1, RAMP2, and RAMP3. Studies have shown that Ramp2 null mice are embryonic lethal, with cardiovascular developmental defects in the heart, blood, and lymphatic vasculatures3.

Alternative names: RAMP2, Receptor activity-modifying protein 2, CRLR activity-modifying protein 2, Calcitonin receptor-like receptor activity-modifying protein 2

Gene ID: 58966

NCBI Accession: NM_005854

UniProt: Q9JHJ1

Pathways: cAMP Metabolic Process, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling