SCTR antibody (Extracellular)

Catalog No. ABIN7043607

Product Details anti-SCTR Antibody

Target: SCTR (Secretin Receptor (SCTR))

Binding Specificity: AA 129-143, Extracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SCTR antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purpose: A Rabbit Polyclonal Antibody to Secretin Receptor

Specificity: Extracellular, N-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: 15 amino acid residues identical,Mouse - 14, human - 12

Characteristics: Anti-Secretin Receptor (extracellular) Antibody (ABIN7043607, ABIN7044414 and ABIN7044415)) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize SCTR from human, mouse, and rat samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: Peptide (C)NSFNERRHAYLLKLK, corresponding to amino acid residues 129 - 143 of rat Secretin Receptor

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: BLP-GR026

Blocking Peptide: BLP-GR026

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for SCTR

Target: SCTR (Secretin Receptor (SCTR))

Alternative Name: SCTR (SCTR Products)

Background: SCTR,Secretin receptor, SCTR, binds Secretin, a hormone that affects water homeostasis throughout the body and specifically in the duodenum of the small intestine.SCTR is ubiquitously expressed throughout the body. It belongs to class B1 of G-protein coupled receptors, along with vasoactive intestinal polypeptide (VIP) receptor. Like all G-protein coupled receptors, it is composed of seven highly conserved transmembrane domains1. The amino terminus of Secretin receptor plays a key role in agonist recognition and responsiveness2.The binding of SCTR to Secretin initiates a signaling cascade that stimulates bicarbonate secretion which in turn leads to neutralization of gastric H+. This process plays an essential role in fat digestion by creating a more neutral ( pH 6 to 8) environment. Secretin levels are regulated by H+ and fatty acids levels in the duodenum and hence the secretion regulation is tightly bound to its activity3.In addition, SCTR-Secretin interaction plays a major role in the regulation of the secretory activity in the heart, liver and the central nervous system (CNS)3.

Alternative names: SCTR, Secretin Receptor

Gene ID: 6344

NCBI Accession: NM_002980

UniProt: P23811