SCN3A antibody (Intracellular)

Catalog No. ABIN7043643

Product Details anti-SCN3A Antibody

Target: SCN3A (Sodium Channel, Voltage-Gated, Type III, alpha Subunit (SCN3A))

Binding Specificity: AA 511-524, Intracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SCN3A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC), Immunoprecipitation (IP)

Purpose: A Rabbit Polyclonal Antibody to SCN3A (NaV1.3) Channel

Specificity: Intracellular, loop between domains I and II

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse - 12,14 amino acid residues identical

Characteristics: Anti-SCN3A (NaV1.3) Antibody (ABIN7043643, ABIN7045227 and ABIN7045228)) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunoprecipitation, and immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize NaV1.3 from rat, human, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)HLEGNHRADGDRFP, corresponding to amino acid residues 511-524 rat NaV1.3

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IHC

Negative Control: BLP-SC004

Blocking Peptide: BLP-SC004

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for SCN3A

Target: SCN3A (Sodium Channel, Voltage-Gated, Type III, alpha Subunit (SCN3A))

Alternative Name: SCN3A (SCN3A Products)

Background: BIII, Brain type III Na+ channel, Sodium channel protein type 3 subunit alpha,Voltage-gated sodium channels (NaV) are essential for the generation of action potentials and for cell excitability.1 NaV channels are activated in response to depolarization and selectively allow flow of Na+ ions. To date, nine NaV α subunits have been cloned and named NaV1.1-NaV1.9.4-5 The NaV channels are classified into two groups according to their sensitivity to Tetrodotoxin (TTX): TTX-sensitive (NaV1.1, NaV1.2, NaV1.3, NaV1.4, NaV1.6 and NaV1.7) and TTX-resistant (NaV1.5, NaV1.8 and NaV1.9).2-3Mammalian sodium channels are heterotrimers, composed of a central, pore-forming α subunit and two auxiliary β subunits. The expression of the α subunit isoform is developmentally regulated and tissue specific. Sodium channels in the adult central nervous system and heart contain β1 through β4 subunits, whereas sodium channels in adult skeletal muscle have only the β1 subunit.6,7NaV1.3, also known as SCN3A, is highly expressed in embryonic sensory neurons and CNS, but its level dramatically decreases in adult rodents.8 Up-regulation of NaV1.3 channel expression was described in injured neurons and injured spinal cord.9-11

Alternative names: SCN3A (NaV1.3), BIII, Brain type III Na+ channel, Sodium channel protein type 3 subunit alpha

Gene ID: 497770

NCBI Accession: NM_006922

UniProt: P08104