SCN11A antibody (Intracellular)

Catalog No. ABIN7043656

Product Details anti-SCN11A Antibody

Target: SCN11A (Sodium Channel, Voltage-Gated, Type XI, alpha Subunit (SCN11A))

Binding Specificity: AA 1748-1765, Intracellular

Reactivity: Rat

Host: Guinea Pig

Clonality: Polyclonal

Conjugate: This SCN11A antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF)

Purpose: A Guinea Pig Polyclonal Antibody to SCN11A (NaV1.9) Channel

Specificity: Intracellular, C-terminus

Predicted Reactivity: 18 amino acid residues identical, human - 14,Mouse - 15

Characteristics: Guinea pig Anti-SCN11A (Nav1.9) Antibody is directed against an epitope of the rat protein. Guinea pig Anti-SCN11A (NaV1.9) Antibody (#), raised in guinea pigs, can be used in western blot and immunohistochemical applications, and has been designed to recognize NaV1.9 from mouse, rat and human samples. The antigen used to immunize guinea pigs is the same as Anti-SCN11A (NaV1.9) Antibody (ABIN7043657, ABIN7045241 and ABIN7045242)) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: CNGDLSSLDVAKVKVHND, corresponding to amino acid residues 1748-1765 of rat NaV1.9

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: BLP-SC017

Blocking Peptide: BLP-SC017

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for SCN11A

Target: SCN11A (Sodium Channel, Voltage-Gated, Type XI, alpha Subunit (SCN11A))

Alternative Name: SCN11A (SCN11A Products)

Background: PN5, SNS2, NAN, Sodium channel protein type 11 subunit alpha,Voltage-gated Na+ channels (NaV) are essential for the generation of action potentials and for cell excitability1. NaV channels are activated in response to depolarization and selectively allow the flow of Na+ ions. To date, nine NaV α subunits have been cloned and named NaV1.1-1.92,3. Their expression is developmentally and tissue specific.NaV channels are classified into two groups according to their sensitivity to tetrodotoxin (TTX): TTX-sensitive and TTX-resistant channels4,5. TTX-resistant channels have been suggested to play an important role in nociceptive transmission. Two TTX-resistant NaV channels are expressed in dorsal root ganglion (DRG) neurons, NaV1.8 and NaV1.9. The NaV1.9 channel (also called SCN11A, NAN or SNS2) is preferentially expressed in small-diameter DRG neurons4,5.Recently, it has been shown that BDNF activated NaV1.9 channels in the hippocampus suggesting that its expression is not restricted to DRG alone6.

Alternative names: SCN11A (NaV1.9), PN5, SNS2, NAN, Sodium channel protein type 11 subunit alpha

Gene ID: 29701

NCBI Accession: NM_014139

UniProt: O88457