SLC16A3 antibody (Intracellular)

Catalog No. ABIN7043675

Product Details anti-SLC16A3 Antibody

Target: SLC16A3 (Solute Carrier Family 16 (Monocarboxylic Acid Transporters), Member 3 (SLC16A3))

Binding Specificity: AA 447-459, Intracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC16A3 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: A Rabbit Polyclonal Antibody to Monocarboxylate Transporter 4

Specificity: Intracellular, C-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,human - identical

Characteristics: Anti-MCT4 (SLC16A3) Antibody (ABIN7043675, ABIN7044618 and ABIN7044619)) is a highly specific antibody directed against an epitope of the rat Monocarboxylate transporter 4. The antibody can be used in western blot analysis. It has been designed to recognize MCT4 from human, rat, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)REVEHFLKAEPEK, corresponding to amino acid residues 447-459 of rat MCT4

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IP

Negative Control: BLP-MT014

Blocking Peptide: BLP-MT014

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for SLC16A3

Target: SLC16A3 (Solute Carrier Family 16 (Monocarboxylic Acid Transporters), Member 3 (SLC16A3))

Alternative Name: SLC16A3 (SLC16A3 Products)

Background: Monocarboxylate transporter 4, Monocarboxylic acid transporter 4, MOT4,Monocarboxylate transporter 4 (MCT4) belongs to a group of membrane proteins responsible for mediating the transfer of various short chain monocarboxylates including lactate, pyruvate, and ketones across the cell membrane. MCTs also transfer H+ resulting in acidification of the tissue microenvironment, thus playing an important role in the control of intracellular pH by proton-coupled release. MCT4 transports monocarboxylates in a ratio of 1:1 with H+.Transport activity of MCT4 is enhanced by carbonic anhydrase II, which has been suggested to function as a "proton antenna" for MCT41-3.The SLC16 gene family of MCTs comprises 14 members with a structure that contains 12 transmembrane domains. Among MCT members, only MCT1 and MCT4 catalyze the proton-coupled transport of lactate and H+. The distribution of MCTs is different by cell type. MCT4 is highly expressed in glycolytic cells, such as skeletal muscle fibers, astrocytes, leukocytes, chondrocytes, and some mammalian cell lineages.MCT4 is extensively investigated in cancer cells and is considered to be a metabolic target for anticancer therapies2-4.

Alternative names: MCT4 (SLC16A3), Monocarboxylate transporter 4, Monocarboxylic acid transporter 4, MOT4

Gene ID: 80878

NCBI Accession: NM_004207

UniProt: O35910

Pathways: Warburg Effect