SLC19A1 antibody (Extracellular)

Catalog No. ABIN7043690

Product Details anti-SLC19A1 Antibody

Target: SLC19A1 (Solute Carrier Family 19 (Folate Transporter), Member 1 (SLC19A1))

Binding Specificity: AA 412-426, Extracellular

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC19A1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (IHC)

Purpose: A Rabbit Polyclonal Antibody to SLC19A1 Transporter

Specificity: 6th extracellular loop.

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat - 13,15 amino acid residues identical, human - 11

Characteristics: Anti-SLC19A1/RFC1 (extracellular) Antibody (ABIN7043690, ABIN7044293 and ABIN7044294)) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and live cell flow cytometry applications. It has been designed to recognize SLC19A1 from human, rat, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)SDKRGLGLQVRDQFR, corresponding to amino acid residues 412 - 426 of mouse SLC19A1 

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:1200

Application Dilutions Western blot wb: 1:400

Comment:

Negative Control: BLP-FT001

Blocking Peptide: BLP-FT001

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for SLC19A1

Target: SLC19A1 (Solute Carrier Family 19 (Folate Transporter), Member 1 (SLC19A1))

Alternative Name: SLC19A1 (SLC19A1 Products)

Background: Solute Carrier Family 19 Member 1, Reduced Folate Carrier 1, Reduced Folate Transporter 1, RFT1, Intestinal folate carrier 1, IFC-1, Reduced Folate Transporter, CHMD, FOLT, REFC, RFC, RFT-1,Solute Carrier Transporter 19A1, (SLC19A1, RFC1) is a high-capacity, bi-directional transporter of 5-methyl-tetrahydrofolate and thiamine monophosphate1,2. SLC19A1 is characterized by 12 transmembrane domains (TMDs) and cytoplasm oriented N- and C-termini3.Folate is a major nutrient that supports important physiological functions such as DNA synthesis, cell division and substrate methylation. Low folate levels caused by suboptimal intake, transport and cellular utilization of folate, is a risk factor for diseases such as spina bifida, cardiovascular diseases and certain types of cancer1.SLC19A1 is the main folate transporter and it plays a critical role in folate uptake and homeostasis in mammalian cells4. High SLC19A1 transcript levels are detected in liver and placenta, with appreciable levels in other tissues, including kidney, lung, bone marrow, intestine, brain, and portions of the central nervous system5.SLC19A1 is also involved in the uptake of Methotrexate (MTX), one of the leading chemotherapeutic agents with the best demonstrated efficacies against childhood acute lymphoblastic leukemia and hence malfunction of this transporter also effects the efficiency of this type of cancer treatment6.

Alternative names: Solute Carrier Family 19 Member 1, Reduced Folate Carrier 1, Reduced Folate Transporter 1, RFT1, Intestinal folate carrier 1, IFC-1, Reduced Folate Transporter, CHMD, FOLT, REFC, RFC, RFT-1

Gene ID: 20509

NCBI Accession: NM_001205206

UniProt: P41438

Pathways: Dicarboxylic Acid Transport