SLC2A3 antibody (Extracellular)

Catalog No. ABIN7043698

Product Details anti-SLC2A3 Antibody

Target: SLC2A3 (Solute Carrier Family 2 (Facilitated Glucose Transporter), Member 3 (SLC2A3))

Binding Specificity: AA 39-51, Extracellular

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC2A3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc))

Purpose: A Rabbit Polyclonal Antibody to Glucose Transporter 3

Specificity: 1st extracellular loop

Cross-Reactivity (Details): It will not recognize rat or mouse GLUT3.

Predicted Reactivity: Human onlyThis antibody will also recognize the human paralog GLUT14,SLC2A14 (Accession Q8TDB8) Homology - 12,13 amino acid residues identical The GLUT14 gene is specific to humans,has an overall 945% amino acid identity to GLUT3

Characteristics: Anti-Human GLUT3 (extracellular) Antibody (ABIN7043698, ABIN7044499 and ABIN7044500)) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and live cell flow cytometry applications. The antibody has been designed to recognize GLUT3 from human samples only. This antibody will also recognize the human paralog GLUT14 /SLC2A14.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KEFINKTLTDKGN, corresponding to amino acid residues 39 - 51 of human GLUT3

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:500

Comment:

Negative Control: BLP-GT043

Blocking Peptide: BLP-GT043

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for SLC2A3

Target: SLC2A3 (Solute Carrier Family 2 (Facilitated Glucose Transporter), Member 3 (SLC2A3))

Alternative Name: SLC2A3 (SLC2A3 Products)

Background: Glucose transporter 3, Facilitated glucose transporter member 3, SLC2A3,Glucose transporter 3 (GLUT3), encoded by SLC2A3 gene is a glucose transporter, specifically expressed in neurons.GLUT3 is a uniporter carrier, it binds one molecule of substrate at a time and transports it across the membrane. Transport occurs down the substrate concentration gradient, thereby allowing a constant supply of blood sugar for cell's metabolism. The substrate binding site of GLUT3 is alternatively accessible from either side of the membrane by conformational changes of the protein. Thus, it enables both intake and export of glucose, according to glucose levels in and out of the cell1.In the context of cancer, tumor cells often increase their glucose consumption and lactate production. An increasing number of studies are identifying GLUT3 and GLUT1 as major participants in the accelerated metabolism demonstrated by cancer cells. GLUT3 and GLUT1 are also overexpressed in several types of cancer tumors and hence, efforts toward the elucidation of the structure and function of glucose transporters as targets for diagnosis and new cancer therapeutics are invested2.GLUT3 shares a conserved fold that is made of two domains, N- and C-terminal, and six trans-membrane spanning α-helices each. The N- and C-terminal domains are connected by a third domain, comprising five short intracellular α-helices. The overall protein structure has roughly the shape of the letter "V", with a cavity opened towards the extracellular side of the membrane that allows glucose to reach and bind to the substrate binding site3,4.

Alternative names: Glucose transporter 3, Facilitated glucose transporter member 3, SLC2A3

Gene ID: 6515

NCBI Accession: NM_006931

UniProt: P11169

Pathways: Warburg Effect