SLC23A2 antibody (Extracellular)

Catalog No. ABIN7043705

Product Details anti-SLC23A2 Antibody

Target: SLC23A2 (Solute Carrier Family 23 (Nucleobase Transporters), Member 2 (SLC23A2))

Binding Specificity: AA 191-204, Extracellular

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC23A2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunofluorescence (Cultured Cells) (IF (cc))

Purpose: A Rabbit Polyclonal Antibody to SVCT2 Transporter

Specificity: 2nd extracellular loop

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat - identical, human - 11,14 amino acid residues identical

Characteristics: Anti-SVCT2/SLC23A2 (extracellular) Antibody (ABIN7043705, ABIN7045312 and ABIN7045313)) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and indirect live cell flow cytometry applications. It has been designed to recognize SVCT2 from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)EITVANGTAELLEH, corresponding to amino acid residues 191-204 of mouse SVCT2

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IFC

Negative Control: BLP-ST022

Blocking Peptide: BLP-ST022

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for SLC23A2

Target: SLC23A2 (Solute Carrier Family 23 (Nucleobase Transporters), Member 2 (SLC23A2))

Alternative Name: SLC23A2 (SLC23A2 Products)

Background: Sodium-dependent vitamin C transporter 2, Na+/L-Ascorbic acid transporter 2, Solute carrier family 23 member 2,Vitamin C, or ascorbic acid, is an important antioxidant. It also participates in numerous cellular functions such as peptide amidation, myelin formation, synaptic potentiation, and protection against glutamate toxicity. Although it circulates in the plasma in micromolar concentrations, it reaches millimolar concentrations in most tissues. These high concentrations are thought to be generated and maintained by SVCT2, a Na+-dependent transporter1.SVCT2 belongs to the SLC23 family of nucleobase-ascorbate transporters (NAT), that consists of SLCT1, SLCT2 and SLCT3 transporters2. The NAT family shares a structure that contains fourteen transmembrane segments that are organized into a pair of "7 + 7" inverted repeats. The intertwined repeats constitute two distinctive domains, namely the core domain and the gate domain2.Neurons in the central nervous system (CNS) contain some of the highest ascorbic acid concentrations in mammalian tissues, and therefore targeted deletion of SVCT2 in mice causes widespread cerebral hemorrhage and death on post-natal day one. SCVT2 is also related to neurodegenerative diseases such as Alzheimer's, Parkinson's, and Huntington's. The expression of SVCT2 is relatively widespread, where it serves to either deliver ascorbic acid to tissues with high demand of vitamin C for enzymatic reactions or to protect metabolically highly active cells or protect specialized tissues from oxidative stress3.

Alternative names: Sodium-dependent vitamin C transporter 2, Na+/L-Ascorbic acid transporter 2, Solute carrier family 23 member 2, SVCT2, SLC23A2

Gene ID: 54338

NCBI Accession: NM_005116

UniProt: Q9EPR4

Pathways: Skeletal Muscle Fiber Development