SLC25A12 antibody (Intracellular)

Catalog No. ABIN7043706

Product Details anti-SLC25A12 Antibody

Target: SLC25A12 (Slc25a12) (Solute Carrier Family 25 (Mitochondrial Carrier, Aralar), Member 12 (Slc25a12))

Binding Specificity: AA 169-181, Intracellular

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC25A12 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Purpose: A Rabbit Polyclonal Antibody to SLC25A12

Specificity: Intracellular, N-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat,human - identical

Characteristics: Anti-SLC25A12 Antibody (ABIN7043706, ABIN7044837 and ABIN7044838)) is a highly selective antibody directed against an epitope of the mouse protein. The antibody can be used in western blot analysis. It has been designed to recognize AGC1 from human, mouse, and rat samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KDKSKSGMISGLD, corresponding to amino acid residues 169-181 of mouse SLC25A12

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: BLP-NT112

Blocking Peptide: BLP-NT112

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for SLC25A12

Target: SLC25A12 (Slc25a12) (Solute Carrier Family 25 (Mitochondrial Carrier, Aralar), Member 12 (Slc25a12))

Alternative Name: SLC25A12 (Slc25a12 Products)

Background: AGC1, ARALAR1, Solute carrier family 25 member 12, Mitochondrial aspartate glutamate carrier 1, Calcium-binding mitochondrial carrier protein Aralar1, Araceli hiperlarga,SLC25 is a large family of nuclear-encoded transporters embedded in the inner mitochondrial membrane and in a few cases other organelle membranes.SLC2512A is a gene encoding the aspartate/glutamate carrier 1 (AGC1). AGC1 possesses six transmembrane alpha helices and N- and C-termini on the cytosolic side of the membrane1.AGC1 catalyzes an exchange between intra-mitochondrial aspartate and cytosolic glutamate, an important step in urea synthesis. As a component of the malate-aspartate shuttle (MAS), AGC1 also has a role in the transfer of NADH, carrying reducing equivalents from the cytosol into the mitochondrial matrix. AGC1 is expressed predominantly in heart and skeletal muscle, with weaker expression in brain and kidney. AGC1 is the main AGC isoform present in the adult brain, and it is expressed mainly in neurons. As part of the MAS, AGC1 has a role in the physiology of pancreatic β-cells by influencing glucose-induced activation of mitochondrial metabolism and insulin secretion and it is the only AGC isoform expressed in pancreatic islets and β-cells. AGC1 has several Ca2+-binding EF-hand motifs located on its long, hydrophilic amino-terminus which extends into the intermembrane space and its activity is strongly regulated by extra-mitochondrial Ca2+. Dysregulation of cellular Ca2+ homeostasis mediated by AGC1 has been implicated in the pathogenesis of autistic spectrum disorder2.

Alternative names: SLC25A12, AGC1, ARALAR1, Solute carrier family 25 member 12, Mitochondrial aspartate glutamate carrier 1, Calcium-binding mitochondrial carrier protein Aralar1, Araceli hiperlarga

Gene ID: 78830

NCBI Accession: NM_003705

UniProt: Q8BH59

Pathways: Ribonucleoside Biosynthetic Process, Dicarboxylic Acid Transport