SLC28A3 antibody (Extracellular)

Catalog No. ABIN7043709

Product Details anti-SLC28A3 Antibody

Target: SLC28A3 (Solute Carrier Family 28 (Sodium-Coupled Nucleoside Transporter), Member 3 (SLC28A3))

Binding Specificity: AA 432-447, Extracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC28A3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc))

Purpose: A Rabbit Polyclonal Antibody to SLC28A3/CNT3

Specificity: 5th extracellular loop

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: human - 14,16 amino acid residues identical,Mouse - 15

Characteristics: Anti-SLC28A3/CNT3 (extracellular) Antibody (ABIN7043709, ABIN7044807 and ABIN7044808)) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and indirect live cell flow cytometry. It has been designed to recognize CNT3 from human, rat, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: Peptide (C)KITLKNAMKMENGDSR, corresponding to amino acid residues 432 - 447 of rat SLC28A3/CNT3

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:400-1:500

Comment:

Negative Control: BLP-NT063

Blocking Peptide: BLP-NT063

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for SLC28A3

Target: SLC28A3 (Solute Carrier Family 28 (Sodium-Coupled Nucleoside Transporter), Member 3 (SLC28A3))

Alternative Name: SLC28A3 (SLC28A3 Products)

Background: Solute Carrier Family 28 Member 3, Concentrative Na+-Nucleoside Cotransporter 3,Solute carrier family 28 member 3 (SLC28A3, CNT3) is a sodium-dependent, concentrative pyrimidine- and purine-selective transporter. SLC28A3 plays an important role in mediating the cellular entry of a broad array of physiological nucleosides and synthetic anticancer nucleoside analog drugs, in a process that is coupled to the sodium cellular gradient1.CNT3 consists of 13 putative trans-membrane domains and it forms a homo-trimer which is important for its activity as a transporter2,3.SLC28 members are crucial determinants in the response to a variety of anticancer and antiviral nucleoside analogs, as they modulate the entry of these analogs into target tissues. Within this large group of solute carriers, SLC28A is a sub-group which consists of SLC28A1, SLC28A2, and SLC28A3. Of the three members of this sub-family, SLC28A3 has the broadest selectivity and the widest expression profile4.

Alternative names: Solute Carrier Family 28 Member 3, Concentrative Na+-Nucleoside Cotransporter 3, CNT3

Gene ID: 140944

NCBI Accession: NM_022127

UniProt: Q8VIH3