SLC29A2 antibody (AA 273-288)

Catalog No. ABIN7043711

Product Details anti-SLC29A2 Antibody

Target: SLC29A2 (Solute Carrier Family 29 (Nucleoside Transporters), Member 2 (SLC29A2))

Binding Specificity: AA 273-288

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC29A2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC)

Purpose: A Rabbit Polyclonal Antibody to Equilibrative Nucleoside Transporter 2 (SLC29A2)

Specificity: 3rd intracellular loop

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Mouse - identical, human - 14,16 amino acid residues identical

Characteristics: Anti-ENT2 (SLC29A2) Antibody (ABIN7043711 and ABIN7044801)) is a highly specific antibody directed against an epitope of the rat equilibrative nucleoside transporter 2. The antibody can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize ENT2 from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)EPQKPGKPSVFVVFRK, corresponding to amino acid residues 273-288 of rat ENT2

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: BLP-NT052

Blocking Peptide: BLP-NT052

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for SLC29A2

Target: SLC29A2 (Solute Carrier Family 29 (Nucleoside Transporters), Member 2 (SLC29A2))

Alternative Name: SLC29A2 (SLC29A2 Products)

Background: Equilibrative nucleoside transporter 2, Nucleoside transporter ei-type,Nucleosides play other important roles beyond their nucleic acid synthesis building block role. For example, they are involved in energy metabolism, they serve as ligands of purinergic receptors and act as influential signaling molecules1. Being hydrophilic, nucleosides cannot simply diffuse across the plasma membrane in order to exert their various functions, but rather need to be physically transported via nucleoside transporters1,2.Two different transporter families are responsible for transporting nucleosides across the plasma membrane: The Concentrative Nucleoside Transporter proteins (CNT, SLC28 family), which consist of three members, CNT1-3, and act as Na+-dependent symporters1,3, and the Equilibrative Nucleoside Transporter proteins ENT1-4 (ENT, SLC29 family), which mediate Na+-independent facilitated diffusion. ENTs act as bidirectional carriers, responsible for the influx and efflux of substrates1.Structurally, ENT transporters have eleven transmembrane domains with an intracellular N-terminal and an extracellular C-terminal1.The best characterized ENT transporters are ENT1 and ENT2, which, although display a broader range of substrate selectivity, have lower affinities for nucleosides compared to concentrative transporters. They are ubiquitously expressed. For example, ENT1 is expressed in erythrocytes, vascular endothelium, the placenta, brain, heart, liver and colon1,4. ENT2 displays more or less the same expression pattern but in addition, is strongly expressed in skeletal muscle1,5. ENT3 is a lysosomal pH -dependent transporter capable of transporting adenine, and ENT4 also transports adenine at acidic pH . They are also broadly expressed with ENT3 displaying high expression in the placenta and ENT4 in the heart6,7.As mentioned above, nucleosides have a variety of cellular/physiological functions suggesting that transporters responsible for their trafficking may also have functional attributes. Indeed, ENT1 plays a role in proliferation and therefore is responsible for the constitutive trafficking of nucleosides1.There is no evidence that nucleoside transporters are directly involved in pathophysiologies, but they are clinically significant. For example, nucleoside transporters are responsible for the cellular uptake of a number of nucleoside-derived anticancer drugs1.

Alternative names: ENT2 (SLC29A2), Equilibrative nucleoside transporter 2, Nucleoside transporter ei-type

Gene ID: 108348052

NCBI Accession: NM_001532

UniProt: O54699