SLC39A8 antibody (Extracellular) (FITC)

Catalog No. ABIN7043727

Product Details anti-SLC39A8 Antibody (FITC)

Target: SLC39A8 (Solute Carrier Family 39 (Zinc Transporter), Member 8 (SLC39A8))

Binding Specificity: AA 221-234, Extracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC39A8 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Purpose: A Rabbit Polyclonal Antibody to Zinc Transporter ZIP8 Conjugated to the Fluorescent Dye FITC

Specificity: 2nd extracellular loop

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse - identical, Human - 12,14 amino acid residues identical

Characteristics: Anti-ZIP8 (SLC39A8) (extracellular) Antibody (ABIN7043726, ABIN7045350 and ABIN7045351)) is a highly specific antibody directed against an epitope of the rat Zinc transporter ZIP8. The antibody can be used in western blot, immunohistochemistry, and indirect live cell flow cytometry. It has been designed to recognize ZIP8 from human, rat, and mouse samples. \nAnti-ZIP8 (SLC39A8) (extracellular)-FITC Antibody (ABIN7043726, ABIN7045350 and ABIN7045351)-F) is directly conjugated to fluorescein isothiocyanate (FITC). The antibody can be used in immunofluorescent applications such as direct live cell flow cytometry.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KTYGQNDHTHFRND, corresponding to amino acid residues 221-234 of rat ZIP8

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: N/A

Comment:

Negative Control: RIC-001-F

Blocking Peptide: BLP-ZT008

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 50 μL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for SLC39A8

Target: SLC39A8 (Solute Carrier Family 39 (Zinc Transporter), Member 8 (SLC39A8))

Alternative Name: SLC39A8 (SLC39A8 Products)

Background: Zinc transporter ZIP8, Zrt- and Irt-like protein 8, Solute carrier family 39 member 8, BIGM103,Zinc uptake transporter, ZIP8, belongs to the SLC39 transporter family. ZIP transporters are responsible for zinc transport into the cytoplasm across cellular membranes, either influx from the extracellular space or efflux from intracellular organelles. Regulation and maintenance of proper concentration and distribution of cellular zinc nutrient by ZIP transporters are essential to the function, metabolism, growth, proliferation and survival of cells1,2.ZIP structure contains eight predicted transmembrane (TM) domains while the N- and C-termini are extracellular. A long loop region is present between TM3 and TM4 domains, harboring a histidine-rich region. TM4 and TM5 domains are amphipathic and are thought to form a cavity through which metals are transported1.ZIP8 protein is encoded by the SLC39A8 gene and is highly expressed in T cells. ZIP8 also plays an important role at the onset of inflammation2.Zinc deficiency can cause growth retardation, cognitive impairment, metabolic disorders, infertility, dysfunction of the immune system, abnormalities in T-cells, natural killer cells and monocytes, and reduction in antibody formation1,2. Studies exploring knockdown of ZIP8 protein show a reduction of cellular zinc content, impaired mitochondrial function in response to TNF-α and increased cell death2.

Alternative names: ZIP8 (SLC39A8), Zinc transporter ZIP8, Zrt- and Irt-like protein 8, Solute carrier family 39 member 8, BIGM103

Gene ID: 295455

NCBI Accession: NM_022154

UniProt: Q5FVQ0