SLC4A4 antibody (Extracellular)

Catalog No. ABIN7043728

Product Details anti-SLC4A4 Antibody

Target: SLC4A4 (Solute Carrier Family 4, Sodium Bicarbonate Cotransporter, Member 4 (SLC4A4))

Binding Specificity: AA 672-685, Extracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC4A4 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF), Immunochromatography (IC), Live Cell Imaging (LCI)

Purpose: A Rabbit Polyclonal Antibody to NBC1 Transporter

Specificity: 2nd extracellular loop, based on the 10 transmembrane topology.

Cross-Reactivity: Mouse, Rat

Cross-Reactivity (Details): Not recommended for human samples. The antibody will recognize all known rat splice variants

Predicted Reactivity: mouse - 12,14 amino acid residues identical,Rat - 13

Characteristics: Anti-SLC4A4 (NBC1) (extracellular) Antibody is directed against an extracellular epitope of rat NBCe1. Anti-SLC4A4 (NBC1) (extracellular) Antibody (ABIN7043728, ABIN7044817 and ABIN7044818)) can be used in western blot, immunohistochemistry and live cell imaging applications. The antibody was used to probe NBCe1 in living rat PC12 cells. It has been designed to recognize NBCe1 from rat and mouse samples and is not recommended for use in human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KE(S)VKFGGKLVGNN, corresponding to amino acid residues 672-685 of rat NBCe1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:400

Application Dilutions Western blot wb: 1:200-1:400

Comment:

Negative Control: BLP-NT075

Blocking Peptide: BLP-NT075

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for SLC4A4

Target: SLC4A4 (Solute Carrier Family 4, Sodium Bicarbonate Cotransporter, Member 4 (SLC4A4))

Alternative Name: SLC4A4 (SLC4A4 Products)

Background: Electrogenic sodium bicarbonate cotransporter 1, NBCe1, NBC-like protein, Na+/HCO3- cotransporter,SLC4 membrane transporter proteins play important roles in kidney acid-base regulation through their transport of bicarbonate (or carbonate), Na+, Cl-, and (possibly NH4+)1. These transporters differ in their substrate (Na+, Cl-) dependence, charge transport stoichiometry, cell-type and developmental expression, functional regulation, and protein-protein interactions. In mammals, SLC4 proteins are encoded by 10 different genes that share protein sequence homology and are grouped according to their functional properties.In humans, the electrogenic sodium bicarbonate cotransporter NBCe1 is encoded by the SLC4A4 gene2. NBCe1-A has a large N-terminal cytoplasmic region, a lipid embedded transmembrane region, and a C-terminal cytoplasmic tail3. The N-terminal cytoplasmic region is tightly folded and is predicted to form a domain structure, unlike the freely aqueous accessible C-terminal cytoplasmic tail. NBCe1-A is composed of either 10 or 14 transmembrane domains. Based on the 10 transmembrane topology, a large extracellular loop (EL) is present between TM3 and TM4 and contains two glycosylated sites. The oligomeric state of the cotransporter is dimeric and each monomeric subunit has independent transport activity4.Mutations in NBCe1 cause autosomal recessive proximal renal tubular acidosis (pRTA). Patients with NBCe1 mutations have severe pRTA, growth and mental retardation, basal ganglia calcification, cataracts, corneal opacities (band keratopathy), glaucoma, elevated serum amylase and lipase, and defects in the enamel consistent with amelogenesis imperfect3. Mice with loss of NBCe1 have a more severe phenotype with marked volume deletion and decreased survival5.

Alternative names: SLC4A4 (NBC1), Electrogenic sodium bicarbonate cotransporter 1, NBCe1, NBC-like protein, Na+/HCO3- cotransporter

Gene ID: 84484

NCBI Accession: NM_003759

UniProt: Q9JI66