SLC40A1 antibody (AA 261-275)

Catalog No. ABIN7043729

Product Details anti-SLC40A1 Antibody

Target: SLC40A1 (Solute Carrier Family 40 (Iron-Regulated Transporter), Member 1 (SLC40A1))

Binding Specificity: AA 261-275

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC40A1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purpose: A Rabbit Polyclonal Antibody to Ferroportin (SLC40A1)

Specificity: 2nd intracellular loop Note: the structure of Ferroportin is still controversial and there is no defined topology yet.

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,rat - 13,15 amino acid residues identical

Characteristics: Anti-Ferroportin (SLC40A1) Antibody is directed against an epitope of human Ferroportin-1. Anti-Ferroportin (SLC40A1) Antibody (ABIN7043729, ABIN7044546 and ABIN7044547)) can be used in western blot and immunohistochemistry applications. It has been designed to recognize Ferroportin-1 from human, rat, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)EGTHLMGVKDSNIHE, corresponding to amino acid residues 261-275 of human Ferroportin

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:500

Comment:

Negative Control: BLP-IT001

Blocking Peptide: BLP-IT001

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for SLC40A1

Target: SLC40A1 (Solute Carrier Family 40 (Iron-Regulated Transporter), Member 1 (SLC40A1))

Alternative Name: SLC40A1 (SLC40A1 Products)

Background: Ferroportin-1, FPN1, Iron-regulated transporter 1, IREG1, Metal transporter protein, MTP1, SLC11A3, HFE4,Ferroportin (SLC40A1) is an iron transporter suggested to play roles in intestinal iron absorption and cellular iron release. To date, Ferroportin is the only known mammalian iron exporter and is essential for transport of iron from one cell type to another1.Ferroportin has 9-12 transmembrane domains. The topology of the transporter has not been defined with precision and the number of transmembrane domains is still under scrutiny. Studies indicate that the amino terminus of Ferroportin is cytosolic. The location of the carboxyl-terminal, however, is unclear. A second controversial issue regarding Ferroportin structure is whether Ferroportin is a monomer or dimer. Many reports using similar approaches including chromatography, cross-linking and physical techniques such as birefringence, have led to contrasting results2.Ferroportin is found in all the tissues where major iron flows are regulated, including duodenal enterocytes, placental trophoblast, macrophages, and hepatocytes3.Mutations that result in defective Ferroportin transport activity will lead to the phenotype of "classic" Ferroportin disease, which is macrophage iron loading and normal to low transferrin saturation2. Expression of mutant Ferroportin in some cultured cell types results in defects in Ferroportin trafficking where the mutant protein never reaches the cell surface4. In other cell types, the mutant Ferroportin was shown to accumulate at the cell surface but had defective transport activity5.

Alternative names: Ferroportin (SLC40A1), Ferroportin-1, FPN1, Iron-regulated transporter 1, IREG1, Metal transporter protein, MTP1, SLC11A3, HFE4

Gene ID: 30061

NCBI Accession: NM_014585

UniProt: Q9NP59

Pathways: Transition Metal Ion Homeostasis