SLC43A1 antibody (Extracellular)

Catalog No. ABIN7043731

Product Details anti-SLC43A1 Antibody

Target: SLC43A1 (Solute Carrier Family 43, Member 1 (SLC43A1))

Binding Specificity: AA 336-349, Extracellular

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC43A1 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: A Rabbit Polyclonal Antibody to LAT3 Transporter

Specificity: 4th extracellular loop

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: 14 amino acid residues identical,Rat - 13, human - 10

Characteristics: Anti-LAT3/SLC43A1 (extracellular) Antibody (ABIN7043731, ABIN7044859 and ABIN7044860)) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot analysis. It has been designed to recognize SLC43A1 from human, rat, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)GKERETNEQRQKVE, corresponding to amino acid residues 336-349 of mouse LAT3 (SLC43A1)

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200-1:400

Comment:

Negative Control: BLP-NT193

Blocking Peptide: BLP-NT193

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for SLC43A1

Target: SLC43A1 (Solute Carrier Family 43, Member 1 (SLC43A1))

Alternative Name: SLC43A1 (SLC43A1 Products)

Background: Large neutral amino acids transporter small subunit 3, L-type amino acid transporter 3, Solute carrier family 43 member 1,LAT3, (L-type amino acid transporter 3) is an amino acid transporter that belongs to the system L family of membrane transporters.The system L transporter family consists of various transporter subtypes with different characteristics in substrate selectivity and transport properties. It is characterized by a Na+ independent transport and its inhibition by 2-aminobicyclo heptane-2-carboxylicacid (BCH).System L transporters are a major route to provide cells with branched-chain and aromatic amino acids. Transporters that belong to this family are present in the basolateral membrane of epithelial cells and play important roles in the absorption of amino acids through the epithelial cells of the small intestine and renal proximal tubules. They are essential in the permeation of amino acids through the blood-tissue barriers such as blood brain barrier and placenta barrier1.LAT3 consists of seven transmembrane domains with a relatively long extracellular loop with predicted N-linked glycosylation sites between transmembrane domains 1 and 2 and a long intracellular loop with several putative phosphorylation sites between transmembrane domains 6 and 72.LAT3 was shown to be up-regulated in prostate cancer and responsible for the increased uptake of leucine into the tumor cells. Hence, it is an appealing target for cancer therapy3.

Alternative names: Large neutral amino acids transporter small subunit 3, L-type amino acid transporter 3, Solute carrier family 43 member 1

Gene ID: 72401

NCBI Accession: NM_003627

UniProt: Q8BSM7