SLC6A12 antibody (Intracellular)

Catalog No. ABIN7043738

Product Details anti-SLC6A12 Antibody

Target: SLC6A12 (Solute Carrier Family 6 (Neurotransmitter Transporter, Betaine/GABA), Member 12 (SLC6A12))

Binding Specificity: AA 22-35, Intracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC6A12 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: A Rabbit Polyclonal Antibody to Betaine/GABA Transporter 1 (SLC6A12)

Specificity: Intracellular, N-terminus

Cross-Reactivity: Mouse, Rat

Cross-Reactivity (Details): Not recommended for human samples.

Predicted Reactivity: Mouse - 13,14 amino acid residues identical

Characteristics: Anti-BGT-1 (SLC6A12) Antibody (ABIN7043738, ABIN7044465 and ABIN7044466)) is a highly specific antibody directed against an epitope of the rat Betaine/GABA transporter 1. The antibody can be used in western blot analysis. It has been designed to recognize BGT-1 from mouse and rat samples. The antibody is unlikely to recognize the protein from human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)GEMMDQKDKDQVKD, corresponding to amino acid residues 22-35 of rat Betaine/GABA Transporter 1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IHC

Negative Control: BLP-GT004

Blocking Peptide: BLP-GT004

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for SLC6A12

Target: SLC6A12 (Solute Carrier Family 6 (Neurotransmitter Transporter, Betaine/GABA), Member 12 (SLC6A12))

Alternative Name: SLC6A12 (SLC6A12 Products)

Background: Betaine/GABA transporter 1, Na+/Cl--dependent betaine/GABA transporter,γ-aminobutyric acid (GABA) transporters (GATs) are essential regulators of the activity in the GABAergic system through their continuous uptake of the neurotransmitter from the synaptic cleft and extrasynaptic space. Four GABA transporters (GAT1, GAT2, GAT3 and BGT1) have been identified. Betaine/GABA transporter (BGT1, SLC6A12) is a member of the Na+- and Cl--dependent neurotransmitter transporter gene family (SLC6) with a high homology to GAT1 (SLC6A1), GAT2 (SLC6A13) and GAT3 (SLC6A11). They have 12 hydrophobic transmembrane domains (TMDs), a large extracellular loop between TMD3 and 4 with multiple glycosylation sites, and intracellular N- and C-termini1,2.BGT1 expression may vary among species. In the dog, BGT1 mRNA is primarily detected in the kidney while the levels in the brain and liver are considerably lower. The highest BGT1 mRNA levels in the mouse are in the liver, while the highest levels in man are in the kidney followed by the liver3. BGT-1 is also present in the brain, but appears to be at low levels and mostly in the leptomeninges rather than in brain tissue proper4.Homozygous BGT1-deficient mice have normal development and show seizure susceptibility indistinguishable from that in wild-type mice in a variety of seizure threshold models5.

Alternative names: BGT-1 (SLC6A12), Betaine/GABA transporter 1, Na+/Cl--dependent betaine/GABA transporter

Gene ID: 50676

NCBI Accession: NM_003044

UniProt: P48056