SLC6A8 antibody (Extracellular)

Catalog No. ABIN7043739

Product Details anti-SLC6A8 Antibody

Target: SLC6A8 (Solute Carrier Family 6 (Neurotransmitter Transporter, Creatine), Member 8 (SLC6A8))

Binding Specificity: AA 46-60, Extracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC6A8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purpose: A Rabbit Polyclonal Antibody to Creatine Transporter

Specificity: Extracellular, C-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,human - identical

Characteristics: Anti-SLC6A8 Antibody (ABIN7043739, ABIN7044471 and ABIN7044472)) is a highly specific antibody directed against an epitope of the rat Creatine transporter. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize SLC6A8 from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RLAVPPRETWTRQMD, corresponding to amino acid residues 46-60 of rat creatine transporter

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: BLP-GT008

Blocking Peptide: BLP-GT008

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for SLC6A8

Target: SLC6A8 (Solute Carrier Family 6 (Neurotransmitter Transporter, Creatine), Member 8 (SLC6A8))

Alternative Name: SLC6A8 (SLC6A8 Products)

Background: Creatine transporter, Sodium- and chloride-dependent creatine transporter 1, CT1, CRT, CHOT1,Creatine transporter (CRT, SLC6A8) is a member of the superfamily of Na+/Cl--coupled transporters for neurotransmitters and organic osmolytes. Creatine Transporter is responsible transports creatine to the blood-brain barrier and into neurons of the central nervous system (CNS). It plays an important role in the storage and utilization of cellular phosphate-bound energy mediated by creatine kinase. It is essential for maintaining brain creatine levels and central nervous system (CNS) functions1,2.Creatine transporter is expressed in various tissues including brain, retina, skeletal muscle, and heart1. The transporter structure contains 12 membrane-spanning domains, a large extracellular loop containing sites for N-linked glycosylation between the third and fourth transmembrane domains. The amino- and carboxyl-termini of the transporter are intracellular3.Creatine transporter is encoded by the SLC6A8 gene and loss-of-function mutations result in creatine deficiency resulting in cerebral creatine deficiency syndromes (CCDSs). These syndromes are the second-most common cause of chromosome X-linked mental retardation. Creatine concentration in the brain is greatly reduced in these patients and they show various neurodevelopmental disorders, including delays in speech and language development, seizures, mental retardation, autism and epilepsy1.

Alternative names: SLC6A8, Creatine transporter, Sodium- and chloride-dependent creatine transporter 1, CT1, CRT, CHOT1

Gene ID: 50690

NCBI Accession: NM_001142805

UniProt: P28570

Pathways: Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, ER-Nucleus Signaling, Unfolded Protein Response