SLC6A11 antibody (Intracellular)

Catalog No. ABIN7043742

Product Details anti-SLC6A11 Antibody

Target: SLC6A11 (Solute Carrier Family 6 (Neurotransmitter Transporter, GABA), Member 11 (SLC6A11))

Binding Specificity: AA 35-47, Intracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC6A11 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: A Rabbit Polyclonal Antibody to GABA Transporter 3 (GAT-3)

Specificity: Intracellular, N-terminus

Cross-Reactivity: Mouse, Rat

Cross-Reactivity (Details): Not recommended for use with human samples.

Predicted Reactivity: Mouse - identical

Characteristics: Anti-GABA Transporter 3 Antibody (ABIN7043742, ABIN7044463 and ABIN7044464) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize from rat and mouse samples. The antibody will unlikely recognize the protein from human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)REARDKAVHERGH, corresponding to amino acid residues 35-47 of rat GAT-3

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:500

Comment:

Negative Control: (ABIN7237157)

Blocking Peptide: (ABIN7237157)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for SLC6A11

Target: SLC6A11 (Solute Carrier Family 6 (Neurotransmitter Transporter, GABA), Member 11 (SLC6A11))

Alternative Name: SLC6A11 (SLC6A11 Products)

Background: SLC6A11, GABT3, Na+/Cl- -dependent GABA transporter 3,γ-Aminobutiric acid (GABA) is the major inhibitory neurotransmitter in the mammalian central nervous system. Transmission via GABA can be halted by its reuptake through GABA transporters (GATs). These membrane proteins belong to the super family of slc6 genes which encode transporters responsible for the uptake of dopamine, serotonin, norepinephrine, glycine and GABA in a Na+-dependent manner1,2.To date, four GATs have been identified: GAT-1, GAT-2, GAT-3 and BGT-1. Like all members of the slc6 family, they have twelve transmembrane domains and short intracellular N- and C-termini1. GATs couple the transport of one GABA molecule to 2 Na+ ions and one Cl- ion3.GAT-3 is highly expressed in the brain and is linked to epilepsy4.

Alternative names: GABA Transporter 3 (GAT-3), SLC6A11, GABT3, Na+/Cl- -dependent GABA transporter 3

Gene ID: 79213

NCBI Accession: NM_014229

UniProt: P31647