SLC8A3 antibody (AA 303-315)

Catalog No. ABIN7043756

Product Details anti-SLC8A3 Antibody

Target: SLC8A3 (Solute Carrier Family 8 (Sodium/calcium Exchanger), Member 3 (SLC8A3))

Binding Specificity: AA 303-315

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC8A3 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF)

Purpose: A Rabbit Polyclonal Antibody to Na+/Ca2+ Exchanger 3 (SLC8A3)

Specificity: 3rd intracellular loop

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,human - identical

Characteristics: Anti-NCX3 (SLC8A3) Antibody is directed against an epitope of the rat Na+/Ca2+ exchange protein 3. Anti-NCX3 (SLC8A3) Antibody (ABIN7043756, ABIN7044879 and ABIN7044880)) can be used in western blot and immunohistochemistry applications. It has been designed to recognize NCX3 from human, rat and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)PLEGKEVDESRRE, corresponding to amino acid residues 303-315 of rat NCX3

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: BLP-NX013

Blocking Peptide: BLP-NX013

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for SLC8A3

Target: SLC8A3 (Solute Carrier Family 8 (Sodium/calcium Exchanger), Member 3 (SLC8A3))

Alternative Name: SLC8A3 (SLC8A3 Products)

Background: Na+/Ca2+ exchange protein 3, Sodium/calcium exchanger 3,Ca2+ has proven to be a universal signaling molecule in excitable and non-excitable cells. However, being that its intracellular concentration is 1000 times lower than that of the extracellular milieu, it is important for the cell to keep this ratio for proper function. NCX, a Na+/Ca2+ exchanger is responsible for most of the efflux of Ca2+ from the cell1-3. The NCX transporter is a member of the SLC8 family of solute carriers which in turn belong to the CaCA superfamily1,4,5.NCX-1 is one of three Na+/Ca2+ exchangers (NCX-1, NCX-2, NCX-3) leading to one Ca2+ movement across the plasma membrane in exchange of three Na+ influx. However, the transporter can reverse the direction of the transport if the concentrations of Na+ and Ca2+ change6. The transporter has nine transmembrane domains and intracellular N- and C-terminals. Between tansmembrane domains 5 and 6, the presence of an extra-long intracellular loop, termed the f-loop, is responsible for regulating the activity of NCX-1 via several different mechanisms like ion binding, phosphorylation, etc. The f-loop also has sites which undergo alternative splicing7.Of the three NCX expressed in mammalian cells, NCX-1 is the most widely expressed. Its expression is detected in the heart, brain, and kidney. NCX-1 undergoes alternative splicing in a tissue-dependent manner. The first splice region does not change the overall structure of the protein but rather enables the expression of the gene specific to the tissues which require the expression of the gene. The second splicing site leads to a number of proteins varying in length. NCX-2 expression is much more limited, it is expressed only in neurons. NCX-3 is expressed in skeletal muscle and in some regions of the brain and undergoes alternative splicing in a similar fashion to that of NCX-11,8.Due to their central role in modulating Ca2+ levels in the cell, NCX exchangers are involved in various pathophysiological diseases/disorders such as hypoxia, aging, Alzheimer's disease7, to name a few.

Alternative names: NCX3 (SLC8A3), Na+/Ca2+ exchange protein 3, Sodium/calcium exchanger 3

Gene ID: 140448

NCBI Accession: NM_001130417

UniProt: P70549